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Recombinant Human USP15, His-tagged

Cat.No.: USP15-15881H
Product Overview: Recombinant human USP15 protein, fused to His tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Description: USP15 is a member of the ubiquitin specific protease (USP) family of deubiquitinating enzymes. USP enzymes play critical roles in ubiquitin-dependent processes through polyubiquitin chain disassembly and hydrolysis of ubiquitin-substrate bonds. The protein associates with the COP9 signalosome, and also plays a role in transforming growth factor beta signalling through deubiquitination of receptor-activated SMAD transcription factors. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 2.
Source: E. coli
Species: Human
Tag: His
Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Molecular Mass: 29.5kDa (258aa) confirmed by MALDI-TOF
Protein length: 1-235aa
AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAEGGAA DLDTQRSDIA TLLKTSLRKG DTWYLVDSRW FKQWKKYVGF DSWDKYQMGD QNVYPGPIDN SGLLKDGDAQ SLKEHLIDEL DYILLPTEGW NKLVSWYTLM EGQEPIARKV VEQGMFVKHC KVEVYLTELK LCENGNMNNV VTRRFSKADT IDTIEKEIRK IFSIPDEKET RLWNKYMSNT FEPLNKPDST IQDAGLYQGQ VLVIEQKNED GTWPRGPSTP KKPLEQSC
Purity: >90% by SDS - PAGE
Applications: SDS-PAGE
Storage: Can be stored at 4°C short term. For long term storage, aliquot and store at at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Concentration: 0.5 mg/ml (determined by Bradford assay)
Gene Name: USP15 ubiquitin specific peptidase 15 [ Homo sapiens ]
Official Symbol: USP15
Synonyms: USP15; ubiquitin specific peptidase 15; ubiquitin specific protease 15; ubiquitin carboxyl-terminal hydrolase 15; KIAA0529; UNPH4; ubiquitin thioesterase 15; deubiquitinating enzyme 15; ubiquitin thiolesterase 15; ubiquitin-specific-processing protease 15; UNPH-2; FLJ55073; MGC74854; MGC131982; MGC149838;
Gene ID: 9958
mRNA Refseq: NM_001252079
Protein Refseq: NP_001239008
MIM: 604731
UniProt ID: Q9Y4E8
Chromosome Location: 12q14
Function: cysteine-type endopeptidase activity; peptidase activity; ubiquitin thiolesterase activity; ubiquitin-specific protease activity;

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