Recombinant Human USP15, His-tagged

• Cat.No.: USP15-15881H
• Product Overview: Recombinant human USP15 protein, fused to His tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: USP15 is a member of the ubiquitin specific protease (USP) family of deubiquitinating enzymes. USP enzymes play critical roles in ubiquitin-dependent processes through polyubiquitin chain disassembly and hydrolysis of ubiquitin-substrate bonds. The protein associates with the COP9 signalosome, and also plays a role in transforming growth factor beta signalling through deubiquitination of receptor-activated SMAD transcription factors. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 2.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
• Molecular Mass: 29.5kDa (258aa) confirmed by MALDI-TOF
• Protein length: 1-235aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAEGGAA DLDTQRSDIA TLLKTSLRKG DTWYLVDSRW FKQWKKYVGF DSWDKYQMGD QNVYPGPIDN SGLLKDGDAQ SLKEHLIDEL DYILLPTEGW NKLVSWYTLM EGQEPIARKV VEQGMFVKHC KVEVYLTELK LCENGNMNNV VTRRFSKADT IDTIEKEIRK IFSIPDEKET RLWNKYMSNT FEPLNKPDST IQDAGLYQGQ VLVIEQKNED GTWPRGPSTP KKPLEQSC
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term. For long term storage, aliquot and store at at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: USP15 ubiquitin specific peptidase 15 [ Homo sapiens ]
• Official Symbol: USP15
• Synonyms: USP15; ubiquitin specific peptidase 15; ubiquitin specific protease 15; ubiquitin carboxyl-terminal hydrolase 15; KIAA0529; UNPH4; ubiquitin thioesterase 15; deubiquitinating enzyme 15; ubiquitin thiolesterase 15; ubiquitin-specific-processing protease 15; UNPH-2; FLJ55073; MGC74854; MGC131982; MGC149838
• Gene ID: 9958
• mRNA Refseq: NM_001252079
• Protein Refseq: NP_001239008
• MIM: 604731
• UniProt ID: Q9Y4E8
• Chromosome Location: 12q14
• Function: cysteine-type endopeptidase activity; peptidase activity; ubiquitin thiolesterase activity; ubiquitin-specific protease activity