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Active Recombinant Human ATF2, GST-tagged

Cat.No. : ATF2-692H
Product Overview : Recombinant Human ATF2, GST-tagged protein (aa19-aa96) is isolated from an E. coli strain that carries the coding sequence of the human ATF2 (19-96) under the control of a T7 promoter.
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  • Gene Information
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Description : The activating transcription factor 2 (ATF2), known as CRE-BP1 (cAMP-responsive element binding protein 1), is a member of the leucine zipper family of DNA binding proteins and binds to both AP-1 and CRE DNA response elements of many viral and cellular promoters. ATF2 is activated by diverse cellular stresses such as inflammatory cytokines, genotoxic agents and UV irradiation. It is activated through phosphorylation at two phospho sites of Thr69 and Thr71 by upstream activators like JNK/p38 (MAPK14, SAPK2A). GST-ATF2 (aa19-aa96) is preferentially used as a useful substrate for MAPK14 and was assayed using MAPK14 (P5049 and R5048) by luminescence-based ATP depletion assay.
Source : E. coli
Species : Human
Tag : GST
Bio-activity : 20 units are sufficient for a gel-mobility shift assay and 100 units are sufficient for a protein-protein interactions assay.
Molecular Mass : 37.1 kDa
Purity : Greater than 95% as determined by SDS-PAGE.
Applications : It can be applied in vitro phosphorylation assay, DNA and protein-protein interactions assays.
Storage : Stored at -70°C before use. Avoid repeated freeze thaw cycles.
Concentration : 2 mg/ml
Storage Buffer : 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA.
Gene Name : ATF2 activating transcription factor 2 [ Homo sapiens ]
Official Symbol : ATF2
Synonyms : ATF2; activating transcription factor 2; cAMP responsive element binding protein 2 , CREB2; cyclic AMP-dependent transcription factor ATF-2; CRE BP1; HB16; TREB7; CREB-2; cAMP-dependent transcription factor ATF-2; cAMP-responsive element-binding protein 2; cAMP response element-binding protein CRE-BP1; cyclic AMP-responsive element-binding protein 2; cAMP responsive element binding protein 2, formerly; activating transcription factor 2 splice variant ATF2-var2; CREB2; CRE-BP1; MGC111558;
Gene ID : 1386
mRNA Refseq : NM_001256090
Protein Refseq : NP_001937
MIM : 602748
UniProt ID : Q16828
Chromosome Location : 12q22-q23
Pathway : Activated TLR4 signalling, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; ERK/MAPK targets, organism-specific biosystem; ERKs are inactivated, organism-specific biosystem; ErbB1 downstream signaling, organism-specific biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem;
Function : MAP kinase tyrosine/serine/threonine phosphatase activity; hydrolase activity; protein tyrosine phosphatase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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