|Cat. No. :
|Product Overview :
||Pro NGF is the pro-form of the neurotrophin nerve growth factor. Like the mature protein pro NGF is characterized by the cysteine knot motif consisting of three cysteine bridges. The protein predominantly exists as a non-covalently linked homodimer. Pro-Nerve Growth Factor Human Recombinant produced in E.Coli is a non-glycosylated, polypeptide chain containing 222 amino acids and having a molecular mass of 49,738 Dalton. The Pro NGF is purified by proprietary chromatographic techniques.
|Physical Appearance :
||Sterile Filtered clear solution.
|Amino acid sequence :
||MEPHSESNVPAGHTIPQVHWTKLQHSLDTALRRARSAPAAAIAARVAGQTRNITVDPRLFKK RRLRSPRVLFSTQPPREAADTQDLDFEVGGAAPFNRTHRSKRSSSHPIFHRGEFSVCDSV SVWVGDKTTATDIKGKEVMVLGEVNINNSVFKQYFFETKCRDPNPVDSGCRGIDSKHWNS YCTTTHTFVKALTMDGKQAAWRFIRIDTACVCVLSRKAR.
||Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
||The sterile protein solution 1.2mg/ml contains 50mM sodium phosphate buffer pH 7.2. 150mM sodium chloride.
|Biological Activity :
||The activity of the protein can by measured by its stimulating effect on the proliferation of TF1 cells (Chevalier et al. 1994 Blood 83, 1479-85). EC50 130 ± 30 pM (TF1 cell assay).
||Pro-Nerve Growth Factor although stable at 15℃ for a week, should be stored at -20℃. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
||Human Pro-NGF; ProNGF; Pro-Nerve Growth Factor.