Recombinant Human CYP46A1, His-tagged

• Cat.No.: CYP46A1-11786H
• Product Overview: Recombinant Human CYP46A1 protein, fused to His-tag, was expressed in E.coli and purified by Ni-sepharose.

Specification

• Description: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein is expressed in the brain, where it converts cholesterol to 24S-hydroxycholesterol. While cholesterol cannot pass the blood-brain barrier, 24S-hydroxycholesterol can be secreted in the brain into the circulation to be returned to the liver for catabolism.
• Source: E.coli
• Species: Human
• Tag: His
• Protein length: C-term-300a.a.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 300mM Imidazole and 0.7% Sarcosyl, 15%glycerol.

Gene Information

• Gene Name: CYP46A1 cytochrome P450, family 46, subfamily A, polypeptide 1 [ Homo sapiens ]
• Official Symbol: CYP46A1
• Synonyms: CYP46A1; cytochrome P450, family 46, subfamily A, polypeptide 1; CYP46, cytochrome P450, subfamily 46 (cholesterol 24 hydroxylase); cholesterol 24-hydroxylase; CH24H; cytochrome P450 46A1; cytochrome P450, subfamily 46 (cholesterol 24-hydroxylase); CP46; CYP46
• Gene ID: 10858
• mRNA Refseq: NM_006668
• Protein Refseq: NP_006659
• MIM: 604087
• UniProt ID: Q9Y6A2
• Chromosome Location: 14q32.1
• Pathway: Bile acid and bile salt metabolism, organism-specific biosystem; Biological oxidations, organism-specific biosystem; Cytochrome P450 - arranged by substrate type, organism-specific biosystem; Endogenous sterols, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; Phase 1 - Functionalization of compounds, organism-specific biosystem
• Function: cholesterol 24-hydroxylase activity; electron carrier activity; heme binding; metal ion binding; monooxygenase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; steroid hydroxylase activity

Reviews

11/24/2022

They can also address any questions or concerns regarding the CYP46A1 protein, thereby facilitating smooth and efficient research execution.

02/21/2017

manufacturers may offer customization options, allowing researchers to tailor the CYP46A1 protein to their specific needs.

05/08/2016

This may include options like different formulations, protein modifications, or tagging techniques. Customization can enhance the versatility of the protein and optimize its utility in the intended research.

Q&As

Can CYP46A1 protein be used as a biomarker for neurodegenerative diseases?

Research suggests that altered CYP46A1 activity and expression may serve as a biomarker for neurodegenerative diseases and could aid in early detection and monitoring of disease progression.

How does CYP46A1 protein influence the development and progression of Huntington's disease?

Dysregulation of CYP46A1-mediated cholesterol metabolism has been implicated in the pathogenesis of Huntington's disease, making it a potential therapeutic target.

Can CYP46A1 protein be targeted for therapeutic interventions in neurodegenerative diseases?

Research is investigating the potential of targeting CYP46A1 for therapeutic interventions, such as the development of small molecule modulators to regulate its activity.

How does CYP46A1 protein impact the efficacy of cholesterol-lowering drugs?

CYP46A1 activity influences the clearance of cholesterol from the brain, which may interact with the effects of cholesterol-lowering drugs, potentially affecting their efficacy.

Are there any genetic variations in the CYP46A1 gene associated with clinical conditions?

Some genetic variations in the CYP46A1 gene have been linked to altered CYP46A1 activity and an increased risk of neurodegenerative diseases.