Recombinant Human PCBP1, His-tagged

• Cat.No.: PCBP1-2314H
• Product Overview: Recombinant human PCBP1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: PCBP1 appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promotes poliovirus RNA replication by binding to its 5"-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. PCBP1 is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
• Molecular Mass: 19.6 kDa
• Protein length: 186aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMDAGVTE SGLNVTLTIR LLMHGKEVGS IIGKKGESVK RIREESGARI NISEGNCPER IITLTGPTNA IFKAFAMIID KLEEDINSSM TNSTAASRPP VTLRLVVPAT QCGSLIGKGG CKIKEIREST GAQVQVAGDM LPNSTERAIT IAGVPQSVTE CVKQIC
• Purity: >85% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml

Gene Information

• Gene Name: PCBP1 poly(rC) binding protein 1 [ Homo sapiens ]
• Official Symbol: PCBP1
• Synonyms: PCBP1; poly(rC) binding protein 1; poly(rC)-binding protein 1; heterogeneous nuclear ribonucleoprotein E1; hnRNP E1; hnRNP X; HNRPE1; HNRPX; alpha-CP1; nucleic acid-binding protein SUB2.3; heterogenous nuclear ribonucleoprotein X; heterogenous nuclear ribonucleoprotein E1; hnRNP-X; hnRNP-E1
• Gene ID: 5093
• mRNA Refseq: NM_006196
• Protein Refseq: NP_006187
• MIM: 601209
• UniProt ID: Q15365
• Chromosome Location: 2p13-p12
• Pathway: Gene Expression, organism-specific biosystem; Processing of Capped Intron-Containing Pre-mRNA, organism-specific biosystem; Spliceosome, organism-specific biosystem; Spliceosome, conserved biosystem; mRNA Splicing, organism-specific biosystem; mRNA Splicing - Major Pathway, organism-specific biosystem
• Function: DNA binding; RNA binding; protein binding; single-stranded DNA binding