Recombinant Human RNF7, His-tagged

• Cat.No.: RNF7-2286H
• Product Overview: Recombinant human RNF7 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Specification

• Description: RNF7 is a highly conserved ring finger protein. It is an essential subunit of SKP1-cullin/CDC53-F box protein ubiquitin ligases, which are a part of the protein degradation machinery important for cell cycle progression and signal transduction. This protein interacts with, and is a substrate of, casein kinase II (CSNK2A1/CKII). The phosphorylation of this protein by CSNK2A1 has been shown to promote the degradation of IkappaBalpha (CHUK/IKK-alpha/IKBKA) and p27Kip1(CDKN1B). Alternatively spliced transcript variants encoding distinct isoforms have been reported.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M Urea
• Molecular Mass: 15.1 kDa
• Protein length: 136aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMADVEDG EETCALASHS GSSGSKSGGD KMFSLKKWNA VAMWSWDVEC DTCAICRVQV MDACLRCQAENKQEDCVVVW GECNHSFHNC CMSLWVKQNN RCPLCQQDWV VQRIGK
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1.0 mg/ml

Gene Information

• Gene Name: RNF7 ring finger protein 7 [ Homo sapiens ]
• Official Symbol: RNF7
• Synonyms: RNF7; ring finger protein 7; RING-box protein 2; CKBBP1; ROC2; SAG; sensitive to apoptosis; zinc RING finger protein SAG; regulator of cullins 2; rbx2; CKII beta-binding protein 1; sensitive to apoptosis gene protein; sensitive to apoptosis, zinc RING finger protein SAG, regulator of cullins 2
• Gene ID: 9616
• mRNA Refseq: NM_001201370
• Protein Refseq: NP_001188299
• MIM: 603863
• UniProt ID: Q9UBF6
• Chromosome Location: 3q22-q24
• Pathway: ECS complex, organism-specific biosystem; ECS complex, conserved biosystem; Ubiquitin mediated proteolysis, organism-specific biosystem; Ubiquitin mediated proteolysis, conserved biosystem
• Function: NEDD8 ligase activity; copper ion binding; metal ion binding; protein binding; zinc ion binding