||The protein was expressed as 6*his-tagged fusion protein by E.Coli and puri?ed by Ni-sepharose.
||SEP1 localizes to cytoplasmic foci containing a complex of mRNA-degrading enzymes. In addition to mRNA metabolism, yeast Sep1 has been implicated in a variety of nuclear and cytoplasmic functions, including homologous recombination, meiosis, telomere maintenance, and microtubule assembly.
||The protein is stored in PBS bu?er at -20 oC. Repeated freezethaw cycles should be avoided.
||1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 300mM Imidazole and 0.7% Sarcosyl, 15%glycerol.