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Recombinant Human CASP3, His tagged
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| Cat.No. : | CASP3-342H |
| Product Overview : | Recombinant Human CASP3 mature form (Q5IS54) (Ser29-His277), fused with a polyhistidine tag at the C-terminus, was produced in E. coli. |
| Source : | E. coli |
| Species : | Human |
| Tag : | His |
| Predicted N Terminal : | Met |
| Form : | Lyophilized from sterile 20mM HEPES, 100mM NaCl, 1mM EDTA, 10% Sucrose, 0.1% chaps, pH 7.5.Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. |
| Molecular Mass : | The recombinant human caspase3 consists of 259 amino acids and predicts a molecular mass of 29.9 KDa. It migrates as an approximately 17 and 15 KDa band in SDS-PAGE under reducing conditions. |
| Endotoxin : | < 1.0 eu per μg of the protein as determined by the LAL method. |
| Purity : | (48.4+48.1) % as determined by SDS-PAGE |
| Stability : | Samples are stable for up to twelve months from date of receipt at -70oC. |
| Storage : | Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4oC before opening to recover the entire contents. |
| Gene Name : | CASP3 caspase 3, apoptosis-related cysteine peptidase [ Homo sapiens ] |
| Official Symbol : | CASP3 |
| Gene ID : | 836 |
| mRNA Refseq : | NM_004346 |
| Protein Refseq : | NP_004337 |
| MIM : | 600636 |
| UniProt ID : | P42574 |
| Chromosome Location : | 4q34 |
◆ Recombinant Protein
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| CASP3-192H | Recombinant Human CASP3, Met&His-tagged | +Inquiry |
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◆ Lysates
| CASP3-7839HCL | Recombinant Human CASP3 293 Cell Lysate | +Inquiry |
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