| Cat.No. : |
CASP3-342H |
| Product Overview : |
Recombinant Human CASP3 mature form (Q5IS54) (Ser29-His277), fused with a polyhistidine tag at the C-terminus, was produced in E. coli. |
| Source : |
E. coli |
| Species : |
Human |
| Tag : |
His |
| Predicted N Terminal : |
Met |
| Form : |
Lyophilized from sterile 20mM HEPES, 100mM NaCl, 1mM EDTA, 10% Sucrose, 0.1% chaps, pH 7.5.Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. |
| Molecular Mass : |
The recombinant human caspase3 consists of 259 amino acids and predicts a molecular mass of 29.9 KDa. It migrates as an approximately 17 and 15 KDa band in SDS-PAGE under reducing conditions. |
| Endotoxin : |
< 1.0 eu per μg of the protein as determined by the LAL method. |
| Purity : |
(48.4+48.1) % as determined by SDS-PAGE |
| Stability : |
Samples are stable for up to twelve months from date of receipt at -70oC. |
| Storage : |
Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4oC before opening to recover the entire contents. |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.