Cat.No.:	CASP3-342H
Product Overview:	Recombinant Human CASP3 mature form (Q5IS54) (Ser29-His277), fused with a polyhistidine tag at the C-terminus, was produced in E. coli.
Source:	E. coli
Species:	Human
Tag:	His
Predicted N Terminal:	Met
Form:	Lyophilized from sterile 20mM HEPES, 100mM NaCl, 1mM EDTA, 10% Sucrose, 0.1% chaps, pH 7.5.Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
Molecular Mass:	The recombinant human caspase3 consists of 259 amino acids and predicts a molecular mass of 29.9 KDa. It migrates as an approximately 17 and 15 KDa band in SDS-PAGE under reducing conditions.
Endotoxin:	< 1.0 eu per μg of the protein as determined by the LAL method.
Purity:	(48.4+48.1) % as determined by SDS-PAGE
Stability:	Samples are stable for up to twelve months from date of receipt at -70oC.
Storage:	Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:	It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4oC before opening to recover the entire contents.

Gene Name:	CASP3 caspase 3, apoptosis-related cysteine peptidase [ Homo sapiens ]
Official Symbol:	CASP3
Gene ID:	836
mRNA Refseq:	NM_004346
Protein Refseq:	NP_004337
MIM:	600636
UniProt ID:	P42574
Chromosome Location:	4q34

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