Recombinant Cattle CASP3 Protein, His-tagged

• Cat.No.: CASP3-623C
• Product Overview: Recombinant Cattle CASP3 Protein (Ser29-Asp175) with His tag was expressed in E. coli.

Specification

• Description: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage.
• Source: E. coli
• Species: Cattle
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 20.2 kDa; Accurate Molecular Mass: 18 kDa
• Protein length: Ser29-Asp175
• Purity: > 97%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: CASP3 caspase 3 [ Bos taurus (cattle) ]
• Official Symbol: CASP3
• Synonyms: CASP3; caspase 3; caspase-3; CASP-3; caspase 3, apoptosis-related cysteine peptidase; EC 3.4.22.56
• Gene ID: 408016
• mRNA Refseq: NM_001077840
• Protein Refseq: NP_001071308
• UniProt ID: Q08DY9

Reviews

03/29/2024

Casp3-748R, the rat caspase3 worked/is working very well for us.