Recombinant Cynomolgus ACE2 protein, His-tagged
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|Product Overview :||Recombinant Cynomolgus ACE2 protein (Gln18-Thr741), fused to His-tag at the N-terminus, was expressed in HEK293.|
|Description :||Angiotensin-converting enzyme 2 (ACE2) is also known as ACEH (ACE homolog), is an integral membrane protein with considerable homologous to ACE, which belongs to the peptidase M2 family. ACE2 is an exopeptidase that catalyses the conversion of angiotensin I to the nonapeptide angiotensin, or the conversion of angiotensin II to angiotensin 1-7. ACE2 may be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, ACE-2 serve as functional receptor for the spike glycoprotein of both coronaviruses. ACE2 is activated by chloride and fluoride, but not bromide and Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. ACE2 is active from pH 6 to 9, and the optimum pH is 6.5 in the presence of 1 M NaCl.|
|Predicted N Terminal :||His|
|Form :||Delivered as bulk protein in a 0.2 μm filtered solution of 50 mM Tris, 150 mM NaCl, Arginine, pH7.5 with glycerol as protectant.|
|Molecular Mass :||The protein has a calculated MW of 85.7 kDa. The protein migrates as 90-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Protein length :||Gln18-Thr741|
|Endotoxin :||Less than 1.0 EU per μg by the LAL method.|
|Purity :||>95% as determined by SDS-PAGE.|
|Storage :||Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
The product MUST be stored at -70 centigrade or lower upon receipt;
-70 centigrade for 3 months under sterile conditions.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|Shipping :||This product is supplied as sterile liquid solution and shipped frozen with dry ice.|
|SDS-PAGE of ACE2-108C :||
Cynomolgus ACE2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
|Bioactivity-ELISA of ACE2-108C :||
Immobilized Cynomolgus ACE2, His Tag at 2 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 S protein RBD, His,Avitag with a linear range of 2-78 ng/mL (QC tested).
Immobilized Cynomolgus ACE2, His Tag at 2 μg/mL (100 μL/well) can bind Biotinylated COVID-19 S1 protein, His,Avitag with a linear range of 0.005-0.313 μg/mL (Routinely tested).
Immobilized Cynomolgus ACE2, His Tag at 2 μg/mL (100 μL/well) can bind COVID-19 S1 protein, Fc Tag with a linear range of 0.4-13 ng/mL (Routinely tested).
Immobilized Cynomolgus ACE2, His Tag at 2 μg/mL (100 μL/well) can bind COVID-19 S protein RBD, Fc Tag with a linear range of 0.2-2 ng/mL (Routinely tested).
|Bioactivity-BLI of ACE2-108C :||
Loaded COVID-19 S protein RBD, Fc Tag on Protein A Biosensor, can bind Cynomolgus ACE2, His Tag with an affinity constant of 4.32 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded COVID-19 S1 protein, Mouse IgG2a Fc Tag on Protein A Biosensor, can bind Cynomolgus ACE2, His Tag with an affinity constant of 3.06 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
|Gene Name :||ACE2|
|Official Symbol :||ACE2|
|Synonyms :||angiotensin I converting enzyme 2|
|Gene ID :||102130864|
|mRNA Refseq :||XM_005593037.2|
|Protein Refseq :||XP_005593094.1|
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◆ Assay kits
|Kit-2210||Angiotensin II Converting Enzyme (ACE2) Activity Assay Kit (Fluorometric)||+Inquiry|
|Kit-2101||Angiotensin II Converting Enzyme Inhibitor Screening Kit||+Inquiry|