||Recombinant Human α-(1,3)-Fucosyltransferase/FUT9 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Thr33-Asn359) of Human FUT9 fused with a polyhistidine tag at the C-terminus.
||α-(1,3)-Fucosyltransferase (FUT9) is a member of the glycosyltransferase 10 family. FUT9 is a single-pass type II membrane protein that is highly expressed in the forebrain and stomach. FUT9 transfers a fucose to lacto-N-neotetraose but not to either α2,3-sialyl lacto-N-neotetraose or lacto-N-tetraose. FUT9 can catalyze the last step in the biosynthesis of the Lewis-x antigen, which forms part of the Lewis blood group-related antigens. FUT9 can be upregulated by progesterone and ovary hormones.
||TNSWIFSPMESASSVLKMKNFFSTKTDYFNETTILVWVWPFGQTFDLTSCQAMFNIQGCHLTTDR SLYNKSHAVLIHHRDISWDLTNLPQQARPPFQKWIWMNLESPTHTPQKSGIEHLFNLTLTYRRDS DIQVPYGFLTVSTNPFVFEVPSKEKLVCWVVSNWNPEHARVKYYNELSKSIEIHTYGQAFGEYVN DKNLIPTISACKFYLSFENSIHKDYITEKLYNAFLAGSVPVVLGPSRENYENYIPADSFIHVEDY NSPSELAKYLKEVDKNNKLYLSYFNWRKDFTVNLPRFWESHACLACDHVKRHQEYKSVGNLEKWF WNLDHHHHHH
||Less than 0.1 ng/μg (1 IEU/μg).
||Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.