Active Recombinant Human JAK1, GST-tagged

• Cat.No.: JAK1-670H
• Product Overview: Human Jak1 (Janus kinase 1), also known as JTK3, GenBank Accession No. NM_002227, a.a. 866-1155(end) with N-terminal GST-Tag, MW= 59 kDa, expressed in Sf9 cells via a baculovirus expression system.

Specification

• Description: Janus kinase 1 (JAK1), is a member of a new class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The second phosphotransferase domain bears all the hallmarks of a protein kinase, although its structure differs significantly from that of the PTK and threonine/serine kinase family members. JAK1 is a large, widely expressed membrane-associated phosphoprotein. JAK1 is involved in the interferon-alpha/beta and -gamma signal transduction pathways. The reciprocal interdependence between JAK1 and TYK2 activities in the interferon-alpha pathway, and between JAK1 and JAK2 in the interferon-gamma pathway, may reflect a requirement for these kinases in the correct assembly of interferon receptor complexes. These kinases couple cytokine ligand binding to tyrosine phosphorylation of various known signaling proteins and of a unique family of transcription factors termed the signal transducers and activators of transcription, or STATs.
• Source: Sf9 cells
• Species: Human
• Tag: GST
• Form: 40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 16 mM glutathione and 20% glycerol.
• Bio-activity: 1.0 pmole/min/μg. Enzyme reaction is conducted at 30°C for 1 hour in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 μM ATP, 0.01% Brij-35 and 2 μM substrate (Tyr6, Invitrogen) using Z-Lyte assay conditions.
• Molecular Mass: 59 kDa
• Purity: ≥ 35%
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Stability: >6 months at –80°C

Gene Information

• Gene Name: JAK1 Janus kinase 1 [ Homo sapiens (human) ]
• Official Symbol: JAK1
• Synonyms: JAK1; JTK3; JAK1A; JAK1B; Janus kinase 1; tyrosine-protein kinase JAK1; EC 2.7.10.2
• Gene ID: 3716
• mRNA Refseq: NM_002227
• Protein Refseq: NP_002218
• MIM: 147795
• UniProt ID: P23458
• Chromosome Location: 1p32.3-p31.3
• Pathway: Antiviral mechanism by IFN-stimulated genes; Cytokine Signaling in Immune system; Epstein-Barr virus infection
• Function: ATP binding; growth hormone receptor binding; non-membrane spanning protein tyrosine kinase activity