Recombinant Canine KITLG protein KITLG-572D

Recombinant Canine KITLG protein

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Recombinant Canine KITLG protein

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Cat.No. : KITLG-572D
Product Overview : Recombinant Canine KITLG protein was expressed in Escherichia coli.
Description : Stem Cell Factor (SCF) which binds to the c-Kit receptor is produced by fibroblasts and endothelial cells. The soluble and transmembrane forms of the protein are formed by alternative splicing of the same RNA transcript and the presence of both soluble and transmembrane SCF is required for normal hematopoietic function. SCF plays an important role in hematopoiesis, spermatogenesis and melanogenesis and it promotes mast cell adhesion, migration, proliferation, and survival. Soluble canine SCF shares 88 %, 93 %, 86 %, 83 %, 76 %, 76 %, 86 % and 88 % a.a. sequence identity with porcine, feline, bovine, human, mouse, rat, goat and equine SCF, respectively. Cells known to express SCF include endothelial cells, fibroblasts and keratinocytes .
Source : E.coli
Species : Canine
Form : Lyophilized from a 0.2μm filtered concentrated solution in 2 × PBS, pH 7.4.
Bio-activity : Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 × 10⁵ IU/mg.
Molecular Mass : Approximately 18.4 kDa, a single non-glycosylated polypeptide chain containing 165 amino acids.
Protein length : 165
AA Sequence : KGICGKRVTDDVKDV TKLVANLPKDYKIAL KYVPGMDVLPSHCWI SVMVEQLSVSLTDLL DKFSNISEGLSNYSI IDKLVKIVDDLVECT EGYSFENVKKAPKSP ELRLFTPEEFFRIFN RSIDAFKDLETVASK SSECVVSSTLSPDKD SRVSVTKPFMLPPVA
Endotoxin : Less than 1 EU/μg of rCaSCF as determined by LAL method.
Purity : >96% by SDS-PAGE and HPLC analysis.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.
SDS-PAGE of KITLG-572D :

Gene Name : KITLG
Official Symbol : KITLG
Synonyms : Hematopoietic Growth Factor KL, MGF
Gene ID : 403507
mRNA Refseq : NM_001012735.2
Protein Refseq : NP_001012753.1
UniProt ID : Q06220

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