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Recombinant Rat Defb1

Cat.No.: Defb1-596R
Product Overview: Recombinant Rat Defb1 produced in E. coli is approximately 4.1 kDa, a single non-glycosylated polypeptide chain containing 37 amino acids.
Description: Defensin are 3-4 kDa antimicrobial peptides of which three distinct families have been identified; α-defensin, β-defensins, and insect defensins. Rat β-Defensin 1, as a member of the β-defensin family, is a salt-sensitive antimicrobial peptide present in epithelia of the lung and urogenital tract. The predicted amino acid sequence shows the hallmark features of other known epithelial β-defensins, including the ordered array of six cysteine residues.
Source: E. coli
Species: Rat
Form: Lyophilized from a 0.2μm filtered concentrated solution in 20 mM PB, 50 0mM NaCl, pH 7.0.
Bio-activity: Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using CD34+ dendritic cells is in a concentration range of 100.0-1000.0 ng/ml.
Molecular Mass: 4.1 kDa
AA Sequence: DQYRCLQNGG FCLRSSCPSH TKLQGTCKPD KPNCCRS
Endotoxin: Less than 1 EU/μg of rRtBD1 as determined by LAL method.
Purity: >95% by SDS-PAGE and HPLC analyses.
Usage: This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Storage: This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 centigrade to -70 centigrade. Avoid repeated freeze/thaw cycles.
Reconstitution: We recommend that this vial be briefy centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions.
Gene Name: Defb1 defensin beta 1 [ Rattus norvegicus ]
Official Symbol: Defb1
Synonyms: DEFB1; defensin beta 1; beta-defensin 1; BD-1; rBD-1;
Gene ID: 83687
mRNA Refseq: NM_031810
Protein Refseq: NP_113998
Function: molecular_function;

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