Recombinant Rat Prom1, His-tagged

• Cat.No.: Prom1-121R
• Product Overview: A DNA sequence encoding the rat PROM1 (F1MAN1) (Asn171-Tyr424) was expressed with a polyhistidine tag at the N-terminus.

Specification

• Description: CD133, also known as PROM1 and Prominin 1, is a pentaspan transmembrane glycoprotein which belongs to the prominin family. It localizes to membrane protrusions and is often expressed on adult stem cells. CD133 is known to play a role in maintaining stem cell properties by suppressing differentiation. CD133 binds cholesterol in cholesterol-containing plasma membrane microdomains. It is proposed to play a role in apical plasma membrane organization of epithelial cells. CD133 is also involved in regulation of MAPK and Akt signaling pathways. Mutations in PROM1 gene have been shown to result in retinitis pigmentosa and Stargardt disease. PROM1 gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Expression of this gene is also associated with several types of cancer.
• Source: Human Cells
• Species: Rat
• Tag: His
• Predicted N Terminal: His
• Form: Lyophilized from sterile PBS, pH 7.4.
• Molecular Mass: The recombinant rat PROM1 comprises 274 amino acids and predicts a molecular mass of 30.9 kDa. The apparent molecular mass of the recombinant protein is approximately 47-57 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Gene Information

• Gene Name: Prom1 prominin 1 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Prom1
• Synonyms: Prom1; Prom; CD133; prominin 1; fudenine
• Gene ID: 60357
• mRNA Refseq: NM_021751
• Protein Refseq: NP_068519
• Chromosome Location: 14q21
• Pathway: Transcriptional misregulation in cancer
• Function: actinin binding; cadherin binding; molecular_function

Reviews

05/01/2021

They can offer detailed characterization data, including purity, stability, and functional assessments, to support researchers' confidence in using the protein in their trials.

10/01/2017

Manufacturers can support researchers in developing assays to measure PROM1 protein levels or activity specific to their clinical trial settings.

12/14/2016

This may involve guidance in selecting appropriate detection methods, providing assay components, or optimizing protocols for optimal performance and sensitivity.

Q&As

Are there specific diseases where PROM1 protein is extensively studied?

Yes, PROM1's role is widely studied in various cancers, including brain, breast, colon, and pancreatic cancers.

What methods are used to study PROM1 protein in clinical settings?

Various laboratory techniques, such as flow cytometry, immunohistochemistry, and molecular biology assays, are employed to study PROM1 expression and function.

Is PROM1 exclusively related to cancer and regenerative medicine?

No, PROM1 is also associated with certain eye disorders and is being researched for potential applications in retinal diseases.

Can PROM1 be a potential target for therapeutic interventions?

Research suggests that it could be a target for treatments aimed at eliminating cancer stem cells and potentially preventing cancer relapse.

Are there any side effects associated with targeting PROM1 in therapies?

The potential impact on normal stem cells and unintended consequences on healthy tissues are areas of concern when targeting PROM1 for therapies.