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Recombinant Rat PROM1 cell lysate

Cat.No. : PROM1-981RCL
Product Overview : Rat CD133 / PROM1 / Prominin 1 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer. Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Rat
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Prom1 prominin 1 [ Rattus norvegicus ]
Official Symbol : PROM1
Synonyms : PROM1; prominin 1; fudenine; Prom; CD133; MGC156650;
Gene ID : 60357
mRNA Refseq : NM_001110137
Protein Refseq : NP_001103607
Pathway : Transcriptional misregulation in cancer, organism-specific biosystem; Transcriptional misregulation in cancer, conserved biosystem;
Function : actinin binding; cadherin binding; molecular_function;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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Are there specific diseases where PROM1 protein is extensively studied? 01/25/2022

Yes, PROM1's role is widely studied in various cancers, including brain, breast, colon, and pancreatic cancers.

What methods are used to study PROM1 protein in clinical settings? 10/24/2021

Various laboratory techniques, such as flow cytometry, immunohistochemistry, and molecular biology assays, are employed to study PROM1 expression and function.

Is PROM1 exclusively related to cancer and regenerative medicine? 01/05/2019

No, PROM1 is also associated with certain eye disorders and is being researched for potential applications in retinal diseases.

Can PROM1 be a potential target for therapeutic interventions? 08/10/2016

Research suggests that it could be a target for treatments aimed at eliminating cancer stem cells and potentially preventing cancer relapse.

Are there any side effects associated with targeting PROM1 in therapies? 03/06/2016

The potential impact on normal stem cells and unintended consequences on healthy tissues are areas of concern when targeting PROM1 for therapies.

Customer Reviews (3)

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Reviews
05/01/2021

    They can offer detailed characterization data, including purity, stability, and functional assessments, to support researchers' confidence in using the protein in their trials.

    10/01/2017

      Manufacturers can support researchers in developing assays to measure PROM1 protein levels or activity specific to their clinical trial settings.

      12/14/2016

        This may involve guidance in selecting appropriate detection methods, providing assay components, or optimizing protocols for optimal performance and sensitivity.

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