Recombinant Xenopus ube2d2, His-tagged

• Cat.No.: ube2d2-236X
• Product Overview: Recombinant full length Xenopus UBE2D2 (UBC4) was expressed in E. coli cells using an N-terminal His tag.

Specification

• Description: UBE2D2(UBC4) Protein or ubiquitin-conjugating enzyme E2D 2 is a member of the E2 ubiquitin-conjugating enzyme family which functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. UBE2D2 specifically ubiquitinates E6AP and in vivo inhibition of UBE2D2 can lead to inhibition of E6-stimulated p53 degradation. UBE2D2 could conjugate ubiquitin to target proteins in an E6AP-dependent manner.
• Source: E. coli
• Species: Xenopus laevis
• Tag: His
• Form: Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
• Molecular Mass: ~17 kDa
• Purity: >95% by densitometry
• Applications: Western Blot
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μl

Gene Information

• Gene Name: ube2d2?ubiquitin-conjugating enzyme E2D 2 (UBC4/5 homolog, yeast) [?Xenopus laevis?(African clawed frog) ]
• Official Symbol: ube2d2
• Synonyms: ube2d2; ubiquitin-conjugating enzyme E2D 2 (UBC4/5 homolog, yeast); EC 6.3.2.-
• Gene ID: 446340
• mRNA Refseq: NM_001093036
• Protein Refseq: ?NP_001086505
• Pathway: Protein processing in endoplasmic reticulum; Ubiquitin mediated proteolysis