Recombinant Human SAE1, GST-tagged SAE1-255H

Cat.No.:	SAE1-255H
Product Overview:	Recombinant full-length human UBLE1A (SAE1) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Description:	UBLE1A (also known as SAE1) or SUMO1 activating enzyme subunit 1 is involved in regulating protein structure and intracellular localization. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins. The SAE1/SAE2 dimer functions in SUMO1 activation in a manner analogous to the single E1 ubiquitin-activating enzymes. The SAE2 inactivation may be a therapeutic strategy in MYC-driven cancers.
Source:	Sf9 insect cells using baculovirus
Species:	Human
Tag:	GST
Form:	Recombinant protein stored in 50mM Tis-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Molecular Mass:	~65 kDa
Purity:	>95% by densitometry
Applications:	Western Blot
Storage:	Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Concentration:	0.1 μg/μl

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Gene Name:	SAE1 SUMO1 activating enzyme subunit 1 [ Homo sapiens ]
Official Symbol:	SAE1
Synonyms:	SAE1; SUMO1 activating enzyme subunit 1; SUMO-activating enzyme subunit 1; activator Of sumo 1; AOS1; FLJ3091; Sua1; activator of SUMO1; SUMO-1 activating enzyme subunit 1; sentrin/SUMO-activating protein AOS1; SUMO-1 activating enzyme E1 N subunit; ubiquitin-like 1-activating enzyme E1A; ubiquitin-like protein SUMO-1 activating enzyme; SUA1; UBLE1A; HSPC140;
Gene ID:	10055
mRNA Refseq:	NM_001145713
Protein Refseq:	NP_001139185
MIM:	613294
UniProt ID:	Q9UBE0
Chromosome Location:	19q13.32
Pathway:	Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination & Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Immune System, organism-specific biosystem; Ubiquitin mediated proteolysis, organism-specific biosystem;
Function:	ATP-dependent protein binding; contributes_to SUMO activating enzyme activity; enzyme activator activity; ligase activity; nucleotide binding; protein C-terminus binding; protein binding; protein heterodimerization activity; protein heterodimerization activity; ubiquitin activating enzyme activity;