"PDPK1" Related Products

Recombinant Human PDPK1, His-tagged

Cat.No.: PDPK1-387H
Product Overview: Recombinant full-length human PDK1 was expressed by baculovirus in Sf9 insect cells using a N-terminal His tag.
Description: PDK1 (3-phosphoinositide-dependent protein kinase) is activated by the presence of PtdIns(3,4,5)P3 or PtdIns(3,4)P2 (1). PDK1 then activates protein kinase B (PKB) which, in turn, inactivates glycogen synthase kinase-3 (GSK3). The phosphorylation of other proteins by PKB and GSK3 is likely to mediate many of the intracellular actions of insulin. Thus, PDK1 plays a key role in mediating many of the actions of the second messenger(s) PtdIns(3,4, 5)P3 and/or PtdIns(3,4)P2. The human PDK1 is a 556-residue monomeric enzyme comprising of a catalytic domain that is most similar to the PKA, PKB and PKC subfamily of protein kinases.
Source: Sf9 insect cells using baculovirus
Species: Human
Tag: His
Form: Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.2mM DTT, 25% glycerol.
Bio-activity: The specific activity of PDK1 was determined to be 118 nmol/min/mg.
Molecular Mass: ~67 kDa
Purity: > 90%
Applications: Kinase Assay; Western Blot
Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Concentration: 0.1 ug/ul
Gene Name: PDPK1 3-phosphoinositide dependent protein kinase-1 [ Homo sapiens (human) ]
Official Symbol: PDPK1
Synonyms: PDPK1; PDK1; PDPK2; PRO0461; 3-phosphoinositide dependent protein kinase-1; 3-phosphoinositide-dependent protein kinase 1; PkB kinase like gene 1; NP_001248745.1; EC 2.7.11.1; NP_002604.1
Gene ID: 5170
mRNA Refseq: NM_002613
Protein Refseq: NP_002604
MIM: 605213
UniProt ID: O15530
Chromosome Location: 16p13.3
Pathway: Activation of NMDA receptor upon glutamate binding and postsynaptic events; Activation of PKB; Adaptive Immune System
Function: 3-phosphoinositide-dependent protein kinase activity; ATP binding; insulin receptor binding

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