Active Recombinant Human PDPK1, His-tagged

• Cat.No.: PDPK1-387H
• Product Overview: Recombinant full-length human PDK1 was expressed by baculovirus in Sf9 insect cells using a N-terminal His tag.

Specification

• Description: PDK1 (3-phosphoinositide-dependent protein kinase) is activated by the presence of PtdIns(3,4,5)P3 or PtdIns(3,4)P2 (1). PDK1 then activates protein kinase B (PKB) which, in turn, inactivates glycogen synthase kinase-3 (GSK3). The phosphorylation of other proteins by PKB and GSK3 is likely to mediate many of the intracellular actions of insulin. Thus, PDK1 plays a key role in mediating many of the actions of the second messenger(s) PtdIns(3,4, 5)P3 and/or PtdIns(3,4)P2. The human PDK1 is a 556-residue monomeric enzyme comprising of a catalytic domain that is most similar to the PKA, PKB and PKC subfamily of protein kinases.
• Source: Sf9 insect cells using baculovirus
• Species: Human
• Tag: His
• Form: Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.2mM DTT, 25% glycerol.
• Bio-activity: The specific activity of PDK1 was determined to be 118 nmol/min/mg.
• Molecular Mass: ~67 kDa
• Purity: > 90%
• Applications: Kinase Assay; Western Blot
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 ug/ul

Gene Information

• Gene Name: PDPK1 3-phosphoinositide dependent protein kinase-1 [ Homo sapiens (human) ]
• Official Symbol: PDPK1
• Synonyms: PDPK1; PDK1; PDPK2; PRO0461; 3-phosphoinositide dependent protein kinase-1; 3-phosphoinositide-dependent protein kinase 1; PkB kinase like gene 1; NP_001248745.1; EC 2.7.11.1; NP_002604.1
• Gene ID: 5170
• mRNA Refseq: NM_002613
• Protein Refseq: NP_002604
• MIM: 605213
• UniProt ID: O15530
• Chromosome Location: 16p13.3
• Pathway: Activation of NMDA receptor upon glutamate binding and postsynaptic events; Activation of PKB; Adaptive Immune System
• Function: 3-phosphoinositide-dependent protein kinase activity; ATP binding; insulin receptor binding