Active Recombinant Mouse Cd14, His-tagged

• Cat.No.: Cd14-118M
• Product Overview: Recombinant Mouse Cd14 was expressed in CHO cells.

Specification

• Description: CD14 acts as a receptor for endotoxin (LPS), is expressed strongly on monocytes, macrophages and weekly at surface of neutrophiles. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor LPS-LBP (lipopolysaccharide binding protein)-complexes. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids, acts at physiological concentrations as LPS agonist and has at higher concentrations an antagonizing effect in cell activation. The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS.?
• Source: CHO cells
• Species: Mouse
• Tag: His
• Form: Lyophilized from a concentrated protein solution (1 mg/ml) containing PBS, pH 7.2
• Bio-activity: Inhibits binding of LPS to CD14. Up to 5μg/ml mouse CD14 inhibit binding of FITC-LPS (0.5μg/ml) to mouse CD14+ CHO transfectants
• Molecular Mass: 50.0 kDa
• AA Sequence: N/A
• Endotoxin: <0.01 ng/ ml
• Purity: 90-95% by SDS-PAGE
• Storage: -20°C
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute in 10 μl of ultra-pure distilled H?O. This solution can then be diluted into other aqueous buffers and stored at –20°C or –70°C for future use

Gene Information

• Gene Name: Cd14?CD14 antigen [Mus musculus (house mouse) ]
• Official Symbol: Cd14
• Synonyms: Cd14; CD14 antigen; monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein
• Gene ID: 12475
• mRNA Refseq: NM_009841
• Protein Refseq: NP_033971
• UniProt ID: P10810
• Chromosome Location: 18 B2; 18 19.46 cM
• Pathway: Amoebiasis; Hematopoietic cell lineage; MAPK signaling pathwa
• Function: lipopolysaccharide binding; lipoteichoic acid binding