|Product Overview:||Recombinant mouse Cd14 (Ala18Pro345) protein with Fc region of human IgG1 at the C-terminus was expressed in mouse myeloma cell line, NS0.|
|Description:||This gene encodes a protein that plays an important role in the innate immune response and is expressed in monocyte/macrophage cells. This gene product acts as a co-receptor that binds several microbial and fungal molecules, including lipopolysaccharides (LPS). This proteins LPS-binding activity is enhanced by the LPS binding protein (LBP) to allow binding to the TLR4-MD-2 co-receptor complex. The product of this gene is found in two forms, either as a soluble protein or attached to the cell surface by a glycosylphosphatidylinositol anchor.|
|Bio-activity:||Measured by its ability to enhance LPS-induced IL-6 secretion by mouse splenocytes. The ED50 for this effect is 0.4-2.4 µg/mL.|
|Molecular Mass:||Predicted Molecular Mass: 61.8 kDa (monomer)
SDS-PAGE: 85-95 kDa, reducing conditions
|N-terminal Sequence Analysis:||Ala18|
|Endotoxin:||<0.01 EU per 1 μg of the protein by the LAL method.|
|Purity:||>95%, by SDS-PAGE under reducing conditions and visualized by silver stain|
|Storage:||Avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Storage Buffer:||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution:||Reconstitute at 1 mg/mL in sterile PBS.|
|Official Symbol:||Cd14 CD14 antigen [ Mus musculus (house mouse) ]|
|Synonyms:||Cd14; CD14 antigen; monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein|