Active Recombinant Human TNFSF13B, His-tagged, Animal Free
Cat.No. : | TNFSF13B-140H |
Product Overview : | Recombinant human BAFF is a glycosylated polypeptide chain containing 151 amino acids (134-285 aa Q9Y275 (TN13B_HUMAN) fused to 10 His tag at N-terminal. rHuman BAFF has a molecular mass between 18 and 20kDa. Human recombinant protein expressed in Nicotiana benthamiana. Recombinant human B lymphocyte activating factor (BAFF) contains a 10-His-tag at the N-terminal end, is produced by transient expression in non-transgenic plants and is purified by sequential chromatography (FPLC). This product contains no animal–derived components or impurities. Animal free product. |
- Specification
- Gene Information
- Related Products
Description : | BAFF (B lymphocyte activating factor) is a member of the tumor necrosis factor (TNF) ligand family which is expressed in T Cells, macrophages, monocytes and dendritic cells. It is also known as BLyS, THANK, TALL, zTNF4 and TNFS20. BAFF enhances B cell survival in vitro and has emerged as a key regulator of peripheric B cell and it is vital homeostatic cytokine for B cells that helps regulate both innate and adaptive immune responses. BAFF binds to three TNF receptors: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium modulator and cyclophilin ligand interactor(TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF 13C).The human BAFF gene code for a 285 amino acids type II transmembrane protein. Recombinant human soluble BAFF is a 151 amino acids containing the TNF-like portion of the extracellular domain of BAFF. |
Source : | Nicotiana benthamiana |
Species : | Human |
Tag : | His |
Form : | Recombinant human BAFF is lyophilized from 20 mM PBS buffer pH 7 and 0.2 M NaCl. |
Bio-activity : | The activity is determined by dose-dependant stimulation of proliferation B cell from Human PBMC. Cell proliferation was measured by MTT method. *activity results may vary with PBMC donors. ED50 ≤ 50ng/ml |
Molecular Mass : | Recombinant human BAFF is a glycosylated polypeptide chain containing 151 amino acids (134-285 aa Q9Y275 (TN13B_HUMAN) fused to 10 His tag at N-terminal. rHuman BAFF has a molecular mass between 18 and 20kDa. |
AA Sequence : | HHHHHHHHHHAVQGPEETVTQDCLQ LIADSETPTIQKGSYTFVPWLLSFK RGSALEEKENKILVKETGYFFIYGQ VLYTDKTYAMGHLIQRKKVHVFGDE LSLVTLFRCIQNMPETLPNNSCYSA GIAKLEEGDELQLAIPRENAQISLD GDVTFFGALKLL |
Endotoxin : | < 0.04 eu ug protein (lal> |
Purity : | >97% by SDS-PAGE gel |
Applications : | Cell culture, Western blot |
Storage : | This lyophilized preparation is stable at 2-8o C for short term, long storage it should be kept at -20oC. Reconstituted protein should be stored in working aliquots at –20°C. Avoid repeated freeze-thaw cycles. |
Reconstitution : | Lyophilized protein should be reconstituted in water to a concentration of 25-50 ng/ul. Upon reconstitution, It can be stored in working aliquots at –20°C for future use. Optimal reconstitution please follow batch Quality Control sheet instructions. |
Gene Name : | TNFSF13B tumor necrosis factor (ligand) superfamily, member 13b [ Homo sapiens ] |
Official Symbol : | TNFSF13B |
Synonyms : | TNFSF13B; tumor necrosis factor (ligand) superfamily, member 13b; TNFSF20; tumor necrosis factor ligand superfamily member 13B; BAFF; BLYS; CD257; TALL 1; TALL1; THANK; delta BAFF; Delta4 BAFF; B-lymphocyte stimulator; B-cell-activating factor; ApoL related ligand TALL-1; TNF homolog that activates apoptosis; dendritic cell-derived TNF-like molecule; tumor necrosis factor-like protein ZTNF4; TNF and ApoL-related leukocyte expressed ligand 1; tumor necrosis factor (ligand) superfamily, member 20; DTL; ZTNF4; TALL-1; |
Gene ID : | 10673 |
mRNA Refseq : | NM_001145645 |
Protein Refseq : | NP_001139117 |
MIM : | 603969 |
UniProt ID : | Q9Y275 |
Chromosome Location : | 13q32-q34 |
Pathway : | Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Intestinal immune network for IgA production, organism-specific biosystem; Intestinal immune network for IgA production, conserved biosystem; Rheumatoid arthritis, organism-specific biosystem; Rheumatoid arthritis, conserved biosystem; |
Function : | cytokine activity; protein binding; receptor binding; tumor necrosis factor receptor binding; |
Products Types
◆ Recombinant Protein | ||
TNFSF13B-097H | Recombinant Human TNFSF13B Protein | +Inquiry |
TNFSF13B-15R | Recombinant Rabbit TNFSF13B Protein | +Inquiry |
TNFSF13B-095H | Recombinant Human TNFSF13B Protein, His-tagged | +Inquiry |
TNFSF13B-299T | Active Recombinant Human TNFSF13B Protein | +Inquiry |
TNFSF13B-0338H | Recombinant Human TNFSF13B Protein (V142-L285), Tag Free | +Inquiry |
◆ Lysates | ||
TNFSF13B-1014CCL | Recombinant Cynomolgus TNFSF13B cell lysate | +Inquiry |
TNFSF13B-2025HCL | Recombinant Human TNFSF13B cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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