||Human TNF-alpha Protein, Biotinylated (Biotinylated TNFa) Val 77 - Leu 233, was labeled with biotin based on rhTNFα Protein, which was produced in human 293 cells (HEK293).?
||This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.?
||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose are added as protectants before lyophilization.
||Biotinylated TNFa, contains no "tag", has a calculated MW of 26 kDa. The predicted N-terminus is Val 77. DTT-reduced Protein migrates as 18kDa.
||Less than 1.0 EU per μg of the Biotinylated TNFa by the LAL method.
||>95% as determined by reduced SDS-PAGE.
||Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).