Recombinant Human APOA1, C13&N15-labeled
Cat.No. : | APOA1-217H |
Product Overview : | Recombinant Human Apo-A1 MS Standard Protein, C13 and N15-labeled (Apo-A1, Heavy Labeled) Asp 25 - Gln 267 (Accession # NP_000030.1) was produced in human 293 cells (HEK293). |
- Specification
- Gene Information
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Description : | ApoA1 is also known as apolipoprotein A-I, ApoA-I , and is the major protein component of high density lipoprotein (HDL) in plasma. It has a specific role in lipid metabolism. Chylomicrons secreted from the intestinal enterocyte also contain ApoA1 but it is quickly transferred to HDL in the bloodstream [1]. The protein promotes cholesterol efflux from tissues to the liver for excretion. It is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. ApoA-I was also isolated as a prostacyclin (PGI2) stabilizing factor, and thus may have an anticlotting effect.[2] Defects in the gene encoding it are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis.[3] In addition, it has been shown that ApoA1 is implicated in the anti-endotoxin function of HDL via interaction with lipopolysaccharide or endotoxin. [4] |
Source : | HEK293 |
Species : | Human |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization. |
Molecular Mass : | Apo-A1, Heavy Labeled, fused with 6×His tag at the C-terminus, has a calculated MW of 29 kDa. The predicted N-terminus is Asp 25. DTT-reduced Protein migrates as 26-30 kDa. |
Endotoxin : | Less than 1.0 EU per μg of the Apo-A1, Heavy Labeled by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. |
Storage : | Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4oC-8oC); After reconstitution under sterile conditions for 1 month (4oC-8oC) or 3 months (-20oC to -70oC). |
Gene Name : | APOA1 apolipoprotein A-I [ Homo sapiens ] |
Official Symbol : | APOA1 |
Synonyms : | APOA1; apolipoprotein A-I; apo-AI; apoA-I; MGC117399; |
Gene ID : | 335 |
mRNA Refseq : | NM_000039 |
Protein Refseq : | NP_000030 |
MIM : | 107680 |
UniProt ID : | P02647 |
Chromosome Location : | 11q23-q24 |
Pathway : | ABC-family proteins mediated transport, organism-specific biosystem; ABCA transporters in lipid homeostasis, organism-specific biosystem; African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Amyloids, organism-specific biosystem; Chylomicron-mediated lipid transport, organism-specific biosystem; Disease, organism-specific biosystem; |
Function : | apolipoprotein A-I receptor binding; apolipoprotein receptor binding; beta-amyloid binding; cholesterol binding; contributes_to cholesterol transporter activity; cholesterol transporter activity; enzyme binding; high-density lipoprotein particle binding; |
Products Types
◆ Recombinant Protein | ||
APOA1-373R | Recombinant Rat APOA1 Protein, His (Fc)-Avi-tagged | +Inquiry |
APOA1-205P | Recombinant Pig APOA1 Protein, His-tagged | +Inquiry |
APOA1-2526H | Recombinant Human APOA1 protein(31-230 aa), C-His-tagged | +Inquiry |
APOA1-1331B | Recombinant Bovine APOA1 Protein (25-265 aa), His-tagged | +Inquiry |
APOA1-53C | Recombinant Cynomolgus Monkey APOA1 Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Native Protein | ||
APOA1-5301H | Native Human Apolipoprotein A-I | +Inquiry |
APOA1-256H | Native Human APOA1 protein | +Inquiry |
ApoA-I-3554H | Native Human ApoA-I | +Inquiry |
◆ Lysates | ||
APOA1-3088HCL | Recombinant Human APOA1 cell lysate | +Inquiry |
APOA1-1497MCL | Recombinant Mouse APOA1 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (17)
Ask a questionMeasurement of APOA1 protein levels is not commonly used as a standalone diagnostic marker for cardiovascular diseases. However, it is often measured as part of lipid profiles to assess the risk of cardiovascular events.
While rare, some genetic mutations can lead to elevated APOA1 protein levels. However, the impact of increased APOA1 levels on overall health and disease risk is not yet fully understood.
APOA1 protein levels are commonly measured along with other lipid markers during cardiovascular risk assessments. However, a single measurement of APOA1 protein levels is not sufficient for diagnosis and is usually interpreted in conjunction with other clinical factors.
Yes, mutations in the APOA1 gene can cause rare genetic disorders such as familial HDL deficiency or Tangier disease. These conditions are characterized by extremely low levels of HDL cholesterol and may lead to premature cardiovascular diseases.
Yes, lifestyle factors can impact APOA1 protein levels and HDL cholesterol levels. Regular exercise, a healthy diet rich in fruits, vegetables, whole grains, and unsaturated fats, can help raise HDL cholesterol levels, potentially increasing APOA1 protein production. Conversely, an unhealthy lifestyle, such as a diet high in saturated fats and sedentary behavior, can lower HDL cholesterol levels.
APOA1 binds to cholesterol and phospholipids in peripheral tissues, such as macrophages. It forms nascent HDL particles, which then accept additional cholesterol from cells. These cholesterol-loaded HDL particles are subsequently transferred to the liver for excretion or recycling.
Yes, variations in APOA1 protein levels and functions have been associated with other health conditions. Some studies suggest a potential link between APOA1 protein deficiency and metabolic disorders, including diabetes and obesity.
The APOA1 protein interacts with several enzymes, receptors, and other proteins involved in lipid metabolism and inflammation. Some examples include interactions with lecithin-cholesterol acyltransferase (LCAT) for cholesterol esterification and with scavenger receptor class B type 1 (SR-B1) for cholesterol uptake in the liver. These interactions play crucial roles in the function and regulation of HDL metabolism.
Recent research suggests that the APOA1 protein may have neuroprotective effects in the brain. It has been associated with decreased risk of neurodegenerative diseases, such as Alzheimer's disease, and may play a role in synaptic function and repair.
Yes, APOA1 is a major component of HDL particles. It plays a crucial role in the formation and maturation of HDL, as well as in the reverse cholesterol transport pathway. Therefore, alterations in APOA1 protein levels or functionality can impact HDL cholesterol levels.
The APOA1 protein possesses anti-inflammatory properties and can modulate immune responses. It is known to inhibit the production of inflammatory cytokines and adhesion molecules, which play a role in the development of atherosclerosis and other inflammatory conditions.
Yes, lifestyle choices can influence APOA1 protein levels. Regular exercise, a healthy diet low in saturated fats, and moderate alcohol consumption have been shown to increase levels of APOA1 protein and improve HDL cholesterol levels.
Researchers are investigating various approaches to boost APOA1 protein levels, such as using synthetic versions of APOA1 or developing drugs that mimic its functions. These strategies aim to enhance reverse cholesterol transport and potentially reduce the risk of cardiovascular diseases.
Various strategies are being explored, including the use of APOA1 mimetic peptides and gene therapy approaches to increase APOA1 protein levels or enhance its functionality. However, these interventions are still in the preclinical or early clinical trial stages.
Dysfunctional APOA1 protein can contribute to the development of familial hypoalphalipoproteinemia, a condition characterized by low levels of high-density lipoprotein (HDL) cholesterol. Low HDL levels are associated with an increased risk of cardiovascular diseases, such as atherosclerosis and coronary artery disease.
APOA1 protein deficiency can lead to impaired reverse cholesterol transport, resulting in increased levels of low-density lipoprotein (LDL) or "bad cholesterol" and decreased levels of HDL. This imbalance is associated with a higher risk of cardiovascular diseases, including atherosclerosis and heart disease.
The liver is the primary site of APOA1 protein synthesis, but it can also be produced by other tissues, such as the intestine. Intestinal APOA1 contributes to the local production of HDL and can influence systemic lipid metabolism.
Customer Reviews (4)
Write a reviewI am highly satisfied with the performance and support associated with this protein, as it has proven to be an essential tool in advancing my scientific investigations.
Its purity and reliability have provided consistent and reliable results in my research.
The prompt and knowledgeable assistance they offer has greatly enhanced my confidence in using the APOA1 protein.
The APOA1 protein is of exceptional quality, making it a perfect fit for my experimental needs.
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