Recombinant Humancytotoxic T-lymphocyte-Associated Protein 4

Cat. No. : CTLA4-137H
Product Overview : Recombinant Human Cytotoxic T-Lymphocyte Associated Antigen-4 produced in insect cells is a homodimeric, glycosylated, polypeptide chain containing a total molecular mass of 80 kDa. Each subunit (40kDa) is fused to a polypeptide linker to the Fc portion of Human IgG1. CTLA4 is expressed in low copy number by T-cells only after activation, but it binds CD28-ligand with approximately 20-fold higher affinity than CD28. A soluble form of the extracellular domain of CTLA-4 has been shown to bind CD28-ligand with high avidity and to suppress T-cell-dependent antibody responses in vivo. Large doses of this soluble protein also suppress responses to a second immunization. CTLA-4 is purified by proprietary chromatographic techniques.
Description : CTLA-4 is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bon. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases.
Source : Sf9 cells.
Amino Acid Sequence : The sequence of the amino acids was determined and was found to be: Lys-Ala-Met-His-Val.
Physical Appearance : Sterile Filtered White lyophilized (freeze-dried) powder.
Purity : Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Formulation : CTLA-4 was lyophilized with no additives.
Solubility : It is recommended to reconstitute the lyophilized Cytotoxic T-Lymphocyte Associated Antigen-4 Human in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Stability : Lyophilized Cytotoxic T-Lymphocyte Associated Antigen Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CTLA-4 should be stored at 4°C between 2-7 days and for future use below -18 °C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Gene Name : CTLA4 cytotoxic T-lymphocyte-associated protein 4 [ Homo sapiens ]
Synonyms : CTLA4; cytotoxic T-lymphocyte-associated protein 4; CD; CD28; CD152; CD_antigen=CD152; CELIAC3; CTLA-4; celiac disease 3; Cytotoxic T-lymphocyte protein 4; cytotoxic T-lymphocyte antigen 4; cytotoxic T-lymphocyte-associated antigen 4; cytotoxic T-lymphocyte-associated serine esterase-4; ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4; ICOS; GSE; ICOS; IDDM12; IDDM12; Short name=CTLA-4; OTTHUMP00000163781;
Gene ID : 1493
mRNA Refseq : NM_001037631
Protein Refseq : NP_001032720
MIM : 123890
UniProt ID : P16410
Chromosome Location : 2q33
Pathway : Autoimmune thyroid disease ; Cell adhesion molecules (CAMs); T cell receptor signaling pathway
Function : Protein binding

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