Active Recombinant Human ADAM17

• Cat.No.: ADAM17-593H
• Product Overview: Recombinant Human ADAM17 was expressed in Insect cells.

Specification

• Description: This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway.
• Source: Insect cells
• Species: Human
• Form: Lyophilised
• Bio-activity: Activity was tested using the synthetic peptide, Abz-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Arg-Dpa(Dnp)-NH2, as a substrate. Specific activity = 280 units/mg.
• Molecular Mass: 62 kDa
• Protein length: 215 to 651
• AA Sequence: RADPDPMKNTCKLLVVADHRFYRYM GRGEESTTTNYLIELIDRVDDIYRN TSWDNAGFKGYGIQIEQIRILKS PQEVKPGEKHYNMAKSYPNEEKDAW DVKMLLEQFSFDIAEEASKVCLAHL FTYQDFDMGTLGLAY VGSPRANSHGGVCPKAYYSPVGKKN IYLNSGLTSTKNYGKTILTKEADLV TTHELGHNFGAEHDP DGLAECAPNEDQGGKYVMYPIAVSG DHENNKMFSNCSKQSIYKTIESKAQ ECFQERSNKVCGNSR VDEGEECDPGIMYLNNDTCCNS DCTLKEGVQC SDRNSPCCKNCQFETAQKKCQEAIN ATCKGVSYCTGNSSECPPPGNAEDD TVCLDLGKCK DGKCIPFCEREQQLESCACNETDNS CKVCCRDLSGRCVPYVDAEQKNLFL RKGKPCTVGF CDMNGKCEKRVQDVIER
• Purity: >90% by SDS-PAGE.
• Applications: Functional Studies; SDS-PAGE
• Storage: Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
• Reconstitution: Reconstitute with sterile deionized water at 0.1µg protein/µl. For long term storage, it is recommended to add a carrier protein (0.1% BSA). Avoid multiple freeze/thaw cycles.

Gene Information

• Gene Name: ADAM17 ADAM metallopeptidase domain 17 [ Homo sapiens (human) ]
• Official Symbol: ADAM17
• Synonyms: ADAM17; CSVP; TACE; NISBD; ADAM18; CD156B; ADAM metallopeptidase domain 17; disintegrin and metalloproteinase domain-containing protein 17; TNF-alpha convertase; snake venom-like protease; TNF-alpha converting enzyme; ADAM metallopeptidase domain 18; tumor necrosis factor, alpha, converting enzyme; NP_003174.3; EC 3.4.24.86
• Gene ID: 6868
• mRNA Refseq: NM_003183
• Protein Refseq: NP_003174
• MIM: 603639
• UniProt ID: P78536
• Chromosome Location: 2p25
• Pathway: Activated NOTCH1 Transmits Signal to the Nucleus; BDNF signaling pathway; Constitutive Signaling by NOTCH1 PEST Domain Mutants
• Function: Notch binding; PDZ domain binding; interleukin-6 receptor binding