Recombinant Human amelotin, His-tagged
Cat.No. : | AMTN-390H |
Product Overview : | AMTN, 17-209aa, Human, His tag, E.coli |
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- Gene Information
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Description : | AMTN belongs to the amelotin family. It could be a cell adhesion protein involved in the maturation of tooth enamel. AMTN is a recently discovered secreted enamel protein predominantly expressed during the maturation stage of enamel formation. It accumulates in a basal lamina-like structure at the interface between ameloblasts and enamel mineral and it co-localizes with another recently described enamel protein, odontogenic ameloblast-associated protein (ODAM). Recombinant human AMTN protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
Source : | E.coli |
Species : | Human |
Tag : | His-tag |
Form : | Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 20% glycerol, 2mM DTT |
Molecular Mass : | 22.2kDa (216aa) confirmed by MALDI-TOF |
AA Sequence : | MGSSHHHHHH SSGLVPRGSH MGSLPQLKPA LGLPPTKLAP DQGTLPNQQQ SNQVFPSLSL IPLTQMLTLG PDLHLLNPAA GMTPGTQTHP LTLGGLNVQQ QLHPHVLPIF VTQLGAQGTI LSSEELPQIF TSLIIHSLFP GGILPTSQAG ANPDVQDGSL PAGGAGVNPA TQGTPAGRLP TPSGTDDDFA VTTPAGIQRS THAIEEATTE SANGIQ |
Purity : | 90>% by SDS - PAGE |
Storage : | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
Concentration : | 0.5mg/ml (determined by Bradford assay) |
Gene Name : | AMTN amelotin [ Homo sapiens ] |
Official Symbol : | AMTN |
Synonyms : | UNQ689 |
Gene ID : | 401138 |
mRNA Refseq : | NM_212557.2 |
Protein Refseq : | NP_997722.1 |
MIM : | 610912 |
UniProt ID : | Q6UX39 |
Chromosome Location : | 4q13.3 |
Function : | molecular_function; |
Products Types
◆ Recombinant Protein | ||
AMTN-2464H | Recombinant Human AMTN Protein, His (Fc)-Avi-tagged | +Inquiry |
AMTN-221H | Recombinant Human AMTN protein(Met1-Gln209), mFc-tagged | +Inquiry |
AMTN-513M | Recombinant Mouse AMTN Protein, His (Fc)-Avi-tagged | +Inquiry |
AMTN-317R | Recombinant Rat AMTN Protein, His (Fc)-Avi-tagged | +Inquiry |
AMTN-661R | Recombinant Rat AMTN Protein | +Inquiry |
◆ Lysates | ||
AMTN-001HCL | Recombinant Human AMTN cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (11)
Ask a questionThe AMTN protein is synthesized in dental cells as a precursor molecule, known as preproamelotin. This precursor undergoes post-translational modifications within the endoplasmic reticulum and Golgi apparatus, including cleavage of the signal peptide, glycosylation, and phosphorylation, to form the mature AMTN protein. The mature AMTN protein is then secreted into the extracellular space where it functions in dental tissue development.
Several studies have identified potential interactions of the AMTN protein with other proteins involved in tooth development and mineralization. For example, AMTN has been reported to interact with the enamelin protein, which is another critical component of enamel formation.
Yes, mutations in the AMTN gene have been linked to a rare genetic disorder called amelogenesis imperfecta. This condition affects the formation and mineralization of dental enamel, leading to abnormal tooth development, enamel defects, and dental abnormalities.
The AMTN protein is primarily expressed in dental tissues, including enamel, dentin, and cementum. However, there is emerging evidence to suggest some low-level expression of AMTN in other tissues. For example, studies have detected AMTN expression in salivary glands, oral mucosa, and certain types of cancer cells. Further research is needed to explore the extent of AMTN expression in non-dental tissues and its potential physiological significance.
Mutations in the AMTN gene have been associated with various types of amelogenesis imperfecta, a group of inherited disorders characterized by defects in enamel formation. These mutations can lead to enamel abnormalities such as thin enamel, enamel loss, enamel hypoplasia (reduced enamel formation), and enamel that is prone to breakdown or staining.
The AMTN protein plays a crucial role in dental tissue development, particularly in the formation and mineralization of enamel. Enamel is the outer layer of the tooth and is the hardest tissue in the human body. AMTN contributes to enamel development by regulating the growth and organization of enamel crystals, promoting their alignment and proper arrangement. AMTN also interacts with other enamel matrix proteins, facilitating their assembly and secretion, ultimately contributing to the formation of a strong and durable enamel layer.
Mutations in the AMTN gene can lead to amelogenesis imperfecta, a condition characterized by abnormal enamel formation. These mutations often disrupt the structure or function of the AMTN protein, leading to impaired enamel maturation, decreased enamel thickness, or loss of enamel formation altogether. These defects can result in enamel that is poorly mineralized, softer than normal, or prone to chipping, resulting in the dental abnormalities observed in amelogenesis imperfecta patients.
The expression of AMTN is regulated by multiple factors and signaling pathways involved in tooth development. Studies have shown that AMTN expression is influenced by transcription factors such as Runx2 and Osterix, which are key regulators of tooth mineralization. Additionally, various signaling pathways, including the Wnt and BMP pathways, appear to be involved in the regulation of AMTN expression.
The AMTN protein is known to contain an N-terminal signal peptide that aids in its secretion from cells. It also has a conserved C-terminal domain known as the "ASAP1 domain" that is characteristic of AMTN proteins. This domain is thought to play a role in protein-protein interactions and the formation of protein complexes.
The AMTN protein has the potential to be used for diagnostic purposes, particularly in identifying genetic mutations associated with amelogenesis imperfecta. Genetic testing for AMTN mutations can help diagnose the condition and provide valuable information for personalized treatment planning.
Several studies have identified functional variations and polymorphisms in the AMTN gene that may have implications for dental health and disease. For example, certain variations in the AMTN gene have been associated with an increased risk of dental caries (tooth decay) and susceptibility to periodontal disease. Additionally, some polymorphisms in the AMTN gene have been linked to variations in enamel thickness and tooth shape.
Customer Reviews (5)
Write a reviewAMTN protein is known for its stability and robustness, making it suitable for a wide range of experimental conditions.
Its resistance to degradation and denaturation allows for longer storage times and repeated use without compromising its performance or functionality.
This specificity enables precise quantification and characterization of AMTN and its interactions with other molecules or proteins of interest.
When used in Western blotting experiments, the AMTN protein produces distinct protein bands, allowing for easy visualization and interpretation.
The AMTN protein exhibits a high degree of specificity in various assays, ensuring accurate and reliable results.
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