Recombinant Human polymerase (RNA) II (DNA directed) polypeptide H, His-tagged

• Cat.No.: POLR2H-556H
• Product Overview: POLR2H, 1-150aa, Human His tag, E.coli

Specification

• Description: POLR2H is one of the essential subunits of RNA polymerase II that is shared by the other two eukaryotic DNA-directed RNA polymerases, I and III. Recombinant human POLR2H protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
• Molecular Mass: 19.5 kDa (173aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAGILFE DIFDVKDIDP EGKKFDRVSR LHCESESFKM DLILDVNIQI YPVDLGDKFR LVIASTLYED GTLDDGEYNP TDDRPSRADQ FEYVMYGKVY RIEGDETSTE AATRLSAYVS YGGLLMRLQG DANNLHGFEV DSRVYLLMKK LAF
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: POLR2H polymerase (RNA) II (DNA directed) polypeptide H [ Homo sapiens ]
• Official Symbol: POLR2H
• Synonyms: POLR2H; polymerase (RNA) II (DNA directed) polypeptide H; DNA-directed RNA polymerases I, II, and III subunit RPABC3; RPB8; hRPB8; RPB8 homolog; DNA-directed RNA polymerase II subunit H; RNA polymerases I, II, and III subunit ABC3; DNA-directed RNA polymerases I, II, and III 17.1 kDa polypeptide; RPB17; RPABC3; hsRPB8
• Gene ID: 5437
• mRNA Refseq: NM_006232
• Protein Refseq: NP_006223
• MIM: 606023
• UniProt ID: P52434
• Chromosome Location: 3q28
• Pathway: Abortive elongation of HIV-1 transcript in the absence of Tat, organism-specific biosystem; Cytosolic DNA-sensing pathway, organism-specific biosystem; Cytosolic DNA-sensing pathway, conserved biosystem; DNA Repair, organism-specific biosystem; Disease, organism-specific biosystem; Dual incision reaction in TC-NER, organism-specific biosystem; Eukaryotic Transcription Initiation, organism-specific biosystem
• Function: DNA-directed RNA polymerase activity; contributes_to protein kinase activity; zinc ion binding