Active Recombinant Feline IL10

• Cat.No.: IL10-215F
• Product Overview: A DNA sequence encoding the feline IL10 (P55029.2) (Ser19-Ile178) was expressed with an initial Met at the N-terminal.

Specification

• Source: E. coli
• Species: Feline
• Form: Lyophilized from sterile PBS, pH7.4.
• Bio-activity: 1. Immobilized felineIL10at 10 μg/ml (100 μl/well) can bind Cynomolgus IL10RA-Fc, The EC50 of Cynomolgus IL10RA-Fc is 0.14-0.32 μg/ml.2. Measured in a cell proliferation assay using MC/9-2 mouse mast cells. The ED50 for this effect is typically 0.1-0.6 n
• Molecular Mass: The recombinant feline IL10 consists of 161 amino acids and has a predicted molecular mass of 18.9 kDa. The apparent molecular mass of it is approximately 19 kDa in SDS-PAGE under reducing conditions.
• Protein length: Ser19-Ile178
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: Hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: IL10?interleukin 10 [?Felis catus?(domestic cat) ]
• Official Symbol: IL10
• Synonyms: IL10; interleukin 10; CSIF; IL-10; cytokine synthesis inhibitory factor
• Gene ID: 493683
• mRNA Refseq: NM_001009209
• Protein Refseq: NP_001009209
• UniProt ID: P55029
• Chromosome Location: F1
• Pathway: African trypanosomiasis; Allograft rejection; Autoimmune thyroid disease