Active Recombinant Human INS

• Cat.No.: INS-320H
• Product Overview: A DNA sequence encoding human insulin B chain (2LGB_B)(Phe1-Lys29) and human insulin A chain (2KXK_A)(Gly1-Asn21) was expressed with a linker AAK.

Specification

• Source: Yeast
• Species: Human
• Form: Lyophilized from sterile PBS, pH7.4.
• Bio-activity: Measured in a serum-free cell proliferation assay using MCF?7 human breast cancer cells.The ED50 for this effect is typically 0.2-1 μg/mL.
• Molecular Mass: The recombinant human Insulin consists of 53 amino acids and predicts a molecular mass of 6 KDa. It migrates as an approximately 6 KDa band in SDS-PAGE under reducing conditions.
• Protein length: Phe1-Lys29 & Gly1-Asn21
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: Hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: INS insulin [ Homo sapiens ]
• Official Symbol: INS
• Synonyms: INS; insulin; proinsulin; ILPR; IRDN; IDDM2; MODY10
• Gene ID: 3630
• mRNA Refseq: NM_000207
• Protein Refseq: NP_000198
• UniProt ID: P01308
• Chromosome Location: 11p15.5
• Pathway: ATF-2 transcription factor network, organism-specific biosystem; Adipogenesis, organism-specific biosystem; Aldosterone-regulated sodium reabsorption, organism-specific biosystem; Aldosterone-regulated sodium reabsorption, conserved biosystem; Amyloids, organism-specific biosystem; Arf6 trafficking events, organism-specific biosystem; Developmental Biology, organism-specific biosystem
• Function: hormone activity; hormone activity; hormone activity; insulin receptor binding; insulin receptor binding; insulin-like growth factor receptor binding; protein binding