Recombinant Human IL13, His-Biotin-tagged

• Cat.No.: IL13-16H
• Product Overview: A DNA sequence encoding the mature form of human IL-13 (Leu25 - Asn146) with 6 His tag on the N-Terminus was expressed in E. Coli. This protein was purified by Ni-NTA column. That protein was conjugated with water soluble biotin.

Specification

• Description: This gene encodes an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4.
• Source: E. Coli
• Species: Human
• Tag: His-Biotin
• Form: Lyophilized 50 ug of human IL-13 Biotinylated in 50 ul of PBS Carry free.
• Molecular Mass: 14 KD
• AA Sequence: Human IL-13 (Leu25 - Asn146) Biotinylated
• Purity: >95%, by SDS-PAGE
• Applications: ELISA
• Storage: Store lyophilized protein at -20 °C or -70 °C. Protect it from light. Lyophilized protein is stable for up to 6 months from date of receipt at - 20 °C to -70 °C. Upon reconstitution, this protein can be stored at -20 °C for a few weeks or at -70 °C in a manual defrost freezer for long term storage (six months). Aliquot reconstituted protein to avoid repeated freezing / thawing cycles.
• Reconstitution: Add 100 ul deionized water to the vial to prepare a working stock solution at 500 ug/mL. Allow to set at least 30 minutes at 4 °C, mix well.

Gene Information

• Gene Name: IL13 interleukin 13 [ Homo sapiens (human) ]
• Official Symbol: IL13
• Synonyms: IL13; P600; IL-13; interleukin 13; interleukin-13
• Gene ID: 3596
• mRNA Refseq: NM_002188
• Protein Refseq: NP_002179
• MIM: 147683
• UniProt ID: P35225
• Chromosome Location: 5q31
• Pathway: Cytokine-cytokine receptor interaction; Cytokines and Inflammatory Response; Fc epsilon RI signaling pathway
• Function: cytokine activity; interleukin-13 receptor binding; protein binding