Recombinant Mouse ABCA1, His-tagged

• Cat.No.: ABCA1-3696M
• Product Overview: ATP Binding Cassette Transporter A1 (ABCA1)

Specification

• Description: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. In humans, this protein functions as a cholesterol efflux pump in the cellular lipid removal pathway. Mutations in the human gene have been associated with Tangier"s disease and familial high-density lipoprotein deficiency.
• Source: E. Coli
• Species: Mouse
• Tag: His
• Form: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Molecular Mass: 30.1kDa
• Protein length: 2261
• Endotoxin: <1.0eu per 1ug (determined by the lal</1.0eu
• Purity: >95%
• Applications: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Notes: Small volumes of ABCA1 recombinant protein may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Warning: This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Gene Information

• Gene Name: Abca1 ATP-binding cassette, sub-family A (ABC1), member 1 [ Mus musculus ]
• Official Symbol: ABCA1
• Synonyms: ABCA1; ATP-binding cassette, sub-family A (ABC1), member 1; ATP-binding cassette sub-family A member 1; ATP-binding cassette 1; ATP-binding cassette transporter 1; Abc1; ABC-1
• Gene ID: 11303
• mRNA Refseq: NM_013454
• Protein Refseq: NP_038482
• Chromosome Location: 4 A5-B3; 4 28.57 cM
• Pathway: ABC transporters, organism-specific biosystem; ABC transporters, conserved biosystem; Fat digestion and absorption, organism-specific biosystem; Fat digestion and absorption, conserved biosystem; HDL-mediated lipid transport, organism-specific biosystem
• Function: ATP binding; ATPase activity; ATPase activity, coupled to transmembrane movement of substances; anion transmembrane transporter activity; apolipoprotein A-I binding; apolipoprotein A-I receptor activity; apolipoprotein binding; cholesterol transporter activity; cholesterol transporter activity; cholesterol transporter activity; nucleoside-triphosphatase activity; nucleotide binding; phospholipid transporter activity; phospholipid transporter activity; phospholipid transporter activity; protein binding; small GTPase binding; syntaxin binding