| Description : |
The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes a catalytic alpha subunit of the gastric H+, K+-ATPase. |
| Source : |
E. Coli |
| Species : |
Human |
| Tag : |
His |
| Form : |
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl. |
| Molecular Mass : |
12.7kDa |
| Protein length : |
1035 |
| Endotoxin : |
<1.0eu per 1ug (determined by the lal</1.0eu |
| Purity : |
>95% |
| Applications : |
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP) |
| Notes : |
Small volumes of ATP4a recombinant protein may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice. |
| Storage : |
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition. |
| Reconstitution : |
Reconstitute in sterile PBS, pH7.2-pH7.4. |
| Warning : |
This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
