|Product Overview :
||Recombinant Human CYP2C19 was expressed in Saccharomyces cerevisiae.
||This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is known to metabolize many xenobiotics, including the anticonvulsive drug mephenytoin, omeprazole, diazepam and some barbiturates. Polymorphism within this gene is associated with variable ability to metabolize mephenytoin, known as the poor metabolizer and extensive metabolizer phenotypes. The gene is located within a cluster of cytochrome P450 genes on chromosome 10q24.
||50 mM Tris (pH 7.4), 1 mM EDTA, 20% glycerol
||activity: ≥2 units/pmol enzymesecondary activity: ≥100 units/mg protein (Reductase activity)
|Unit Definition :
||P450 Activity: One unit will oxidize 1 picomole of diclofenac per minute at pH 7.4 at 30 °C.Reductase Activity: One unit will reduce 1 nanomole of cytochrome C per minute in the presence of NADPH at pH 7.4 at 28 °C.
||≤90 mg/mL (Protein)≥10 pmol/mg protein (P450)