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Recombinant Human GDNF, His-tagged

Cat.No.: GDNF-22H
Product Overview: Recombinant Human GDNF was purified from the endosperm tissue of barley grain (Hordeum vulgare).
Description: Glial cell line derived neurotrophic factor (GDNF) is a protein promoting the survival of various neuronal subpopulations at different stages of development. These include dopaminergic neurons in midbrain, GDNF stimulates their survival, differentiation and high-affinity dopamine uptake. The use of GDNF in the treatment of Parkinson"s disease and spinal cord injuries has shown promising results. GDNF belongs to the TGF-? superfamily, it is a homodimeric protein with the familial cysteine-knot structure. Cells expressing GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons.
Source: Barley grain (Hordeum vulgare)
Species: Human
Tag: His
Form: Sterile filtered through a 0.2 μm filter. Lyophilized from PBS, pH 7.2
Bio-activity: Recombinant human GDNF was determined by its dose dependent effect on proliferation of rat C6 cells and found to be fully comparable to a commercially available GDNF. Optimal concentration should be determined for specific applications and cell lines.
Molecular Mass: Recombinant human GDNF contains 134 amino acids and a 16 a.a. histidine-based tag for a total length of 150 a.a. and has a predicted molecular mass of 17.2 kDa including his-tag. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 25 kDa in SDS-PAGE.
Endotoxin: Endotoxin level is less than 0.005ng per μg of product (0.05EU/μg).
Purity: Greater than 95% by SDS-PAGE gel analysis.
Storage: The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Reconstitution: Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Gene Name: GDNF glial cell derived neurotrophic factor [ Homo sapiens ]
Official Symbol: GDNF
Synonyms: GDNF; glial cell derived neurotrophic factor; glial cell line-derived neurotrophic factor; astrocyte derived trophic factor; ATF1; ATF2; glial cell line derived neurotrophic factor; glial derived neurotrophic factor; HFB1 GDNF; ATF; astrocyte-derived trophic factor; HSCR3; HFB1-GDNF;
Gene ID: 2668
mRNA Refseq: NM_000514
Protein Refseq: NP_000505
MIM: 600837
UniProt ID: P39905
Chromosome Location: 5p13.1-p12
Pathway: Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; NCAM signaling for neurite out-growth, organism-specific biosystem; NCAM1 interactions, organism-specific biosystem; Signaling events regulated by Ret tyrosine kinase, organism-specific biosystem;
Function: growth factor activity; protein homodimerization activity; receptor binding;

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