| Description : |
Stem Cell Factor (SCF, also known as c-kit ligand, mast cell growth factor and steel factor) is a hematopoietic growth factor acting at the early stages of hematopoiesis. SCF promotes the survival, proliferation and differentiation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. It also stimulates the growth and activation of mast cells and eosinophils. SCF has been shown to act synergistically with other growth factors, such as GM-CSF, G-CSF, IL-7 and EPO. Human SCF shows low activity on murine cells, while murine and rat SCF are fully active on human cells. |
| Source : |
Barley grain (Hordeum vulgare) |
| Species : |
Human |
| Tag : |
His |
| Form : |
Sterile filtered through a 0.2 μm filter. Lyophilized from PBS, pH 7.2 |
| Bio-activity : |
Recombinant human SCF is measured by its dose-dependent effect on the proliferation of TF-1 cells. The ED50 for this effect is typically between 2 - 10 ng/ml corresponding to specific activity of 1 – 5 x 10e5 U/mg. We recommend that the optimal concentration for each specific application be determined by an initial dose-response assay. |
| Molecular Mass : |
Recombinant human SCF contains 165 amino acids and a 16 a.a. histidine-based tag for a total length of 181 a.a. and has a predicted molecular mass of 20.7 kDa including his-tag. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 22-24 kDa in SDS-PAGE. |
| Endotoxin : |
Endotoxin level is less than 0.005ng per μg of product (0.05EU/μg) |
| Purity : |
Greater than 95% by SDS-PAGE gel analysis |
| Storage : |
The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). |
