Recombinant Mouse S100A4 cell lysate

• Cat.No.: S100A4-001MCL
• Product Overview: Mouse S100A4 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Human cells
• Species: Mouse
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.

Gene Information

• Gene Name: S100a4 S100 calcium binding protein A4 [ Mus musculus ]
• Official Symbol: S100A4
• Synonyms: S100A4; S100 calcium binding protein A4; protein S100-A4; calvasculin; protein 18A2; protein Mts1; metastatic cell protein; S100 calcium-binding protein A4; placental calcium-binding protein; 42a; 18A2; Capl; FSp1; Mts1; pk9a; PeL98; metastasin
• Gene ID: 20198
• mRNA Refseq: NM_011311
• Protein Refseq: NP_035441
• Function: RAGE receptor binding; calcium ion binding; calcium-dependent protein binding; identical protein binding; protein binding

Reviews

10/07/2022

I have been using these for several years and they have been very stable and I swear by this product.

04/14/2022

The experimental process is simple and there is no special environmental requirement.

11/20/2021

It can remain stable under different temperature and pH conditions.

Q&As

What is the current progress in drug development for S100A4?

Drug development for S100A4 is currently in clinical trials, including small molecule inhibitors against S100A4 and inhibitors against the S100A4 signaling pathway.

Is the expression of S100A4 affected by environmental factors?

Yes, the expression of S100A4 is affected by environmental factors, such as nutritional status, inflammation level, etc.

What role does S100A4 play in tumor immunotherapy?

S100A4 plays an important role in tumor immunotherapy, it can act as a tumor-associated antigen, activate the immune response of tumor cells, promote apoptosis of tumor cells and inhibit the proliferation of tumor cells.

How to improve the nutritional status of S100A4 protein through diet?

There is no conclusive evidence that diet improves the nutritional status of S100A4 protein, but maintaining a balanced diet and moderate protein intake can help maintain good health.

How can S100A4 be used as a biomarker in clinical practice?

In clinical practice, the expression level of S100A4 can be detected to predict disease progression and prognosis, and to guide the choice of treatment regimen. For example, in the diagnosis and treatment of oncological diseases.

What is the relationship between S100A4 protein and muscle wasting?

Studies have shown that S100A4 protein can inhibit the occurrence of muscle wasting, but the specific mechanism is not clear.