Recombinant Mouse F7 cell lysate
Cat.No. : | F7-001MCL |
Product Overview : | Mouse FVII / Factor VII / F7 derived in CHO cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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Source : | CHO cells |
Species : | Mouse |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | F7 coagulation factor VII [ Mus musculus ] |
Official Symbol : | F7 |
Synonyms : | F7; coagulation factor VII; serum prothrombin conversion accelerator; Cf7; FVII; mfVII; AI132620; |
Gene ID : | 14068 |
mRNA Refseq : | NM_010172 |
Protein Refseq : | NP_034302 |
Pathway : | Blood Clotting Cascade, organism-specific biosystem; Complement and Coagulation Cascades, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Extrinsic Pathway, organism-specific biosystem; Formation of Fibrin Clot (Clotting Cascade), organism-specific biosystem; Gamma-carboxylation of protein precursors, organism-specific biosystem; |
Function : | calcium ion binding; catalytic activity; endopeptidase activity; glycoprotein binding; hydrolase activity; peptidase activity; receptor binding; serine-type endopeptidase activity; serine-type peptidase activity; |
Products Types
◆ Recombinant Protein | ||
F7-2206M | Active Recombinant Mouse F7 protein(Met1-Leu446), His-tagged | +Inquiry |
F7-2893H | Recombinant Human F7 Protein, His (Fc)-Avi-tagged | +Inquiry |
F7-3622H | Recombinant Human F7 Protein, GST-tagged | +Inquiry |
F7-911R | Recombinant Rat F7 Protein, His&GST-tagged | +Inquiry |
F7-1837R | Recombinant Rat F7 Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Native Protein | ||
F7-5303H | Native Human Coagulation Factor VII (serum prothrombin conversion accelerator) | +Inquiry |
◆ Lysates | ||
F7-1973HCL | Recombinant Human F7 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionFactor VII's role in the coagulation cascade ensures rapid initiation of clot formation, preventing excessive bleeding and contributing to hemostasis.
Factor VII is a key player in the extrinsic pathway, where it forms a complex with tissue factor to activate factor X, initiating the cascade leading to thrombin generation.
Factor VII is activated through a proteolytic cleavage, converting it into its active form (VIIa). This activation is regulated by tissue factor and other coagulation factors.
F7 deficiency results in impaired blood clotting, leading to a bleeding disorder known as coagulation factor VII deficiency. This condition can manifest in varying degrees of severity.
Factor VII is activated to its enzymatically active form (factor VIIa) and initiates the coagulation cascade by activating factor X, ultimately leading to the formation of a stable blood clot.
Customer Reviews (3)
Write a reviewQuick turnaround—this product arrived swiftly, facilitating our research.
Exceptional quality and precision—our lab relies on this protein consistently.
Invaluable assistance from customer service—professional and responsive.
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