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Recombinant Mouse F7 cell lysate

Cat.No. : F7-001MCL
Product Overview : Mouse FVII / Factor VII / F7 derived in CHO cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : CHO cells
Species : Mouse
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : F7 coagulation factor VII [ Mus musculus ]
Official Symbol : F7
Synonyms : F7; coagulation factor VII; serum prothrombin conversion accelerator; Cf7; FVII; mfVII; AI132620;
Gene ID : 14068
mRNA Refseq : NM_010172
Protein Refseq : NP_034302
Pathway : Blood Clotting Cascade, organism-specific biosystem; Complement and Coagulation Cascades, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Extrinsic Pathway, organism-specific biosystem; Formation of Fibrin Clot (Clotting Cascade), organism-specific biosystem; Gamma-carboxylation of protein precursors, organism-specific biosystem;
Function : calcium ion binding; catalytic activity; endopeptidase activity; glycoprotein binding; hydrolase activity; peptidase activity; receptor binding; serine-type endopeptidase activity; serine-type peptidase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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How does F7 contribute to hemostasis, and what is its role in preventing excessive bleeding? 04/02/2021

Factor VII's role in the coagulation cascade ensures rapid initiation of clot formation, preventing excessive bleeding and contributing to hemostasis.

Discuss the role of F7 in the extrinsic pathway of blood coagulation. 07/20/2020

Factor VII is a key player in the extrinsic pathway, where it forms a complex with tissue factor to activate factor X, initiating the cascade leading to thrombin generation.

What are the mechanisms of F7 activation, and how is it regulated at the molecular level? 01/15/2019

Factor VII is activated through a proteolytic cleavage, converting it into its active form (VIIa). This activation is regulated by tissue factor and other coagulation factors.

Explain the biochemical basis of F7 deficiency and its implications for bleeding disorders. 11/05/2018

F7 deficiency results in impaired blood clotting, leading to a bleeding disorder known as coagulation factor VII deficiency. This condition can manifest in varying degrees of severity.

Explain the role of F7 in the coagulation cascade and blood clot formation. 09/28/2018

Factor VII is activated to its enzymatically active form (factor VIIa) and initiates the coagulation cascade by activating factor X, ultimately leading to the formation of a stable blood clot.

Customer Reviews (3)

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Reviews
05/18/2022

    Quick turnaround—this product arrived swiftly, facilitating our research.

    10/03/2019

      Exceptional quality and precision—our lab relies on this protein consistently.

      05/29/2018

        Invaluable assistance from customer service—professional and responsive.

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