Recombinant Human ALOX15B cell lysate

• Cat.No.: ALOX15B-002HCL
• Product Overview: Human ALOX15B derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Baculovirus-Insect Cells
• Species: Human
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.

Gene Information

• Gene Name: ALOX15B arachidonate 15-lipoxygenase, type B [ Homo sapiens ]
• Official Symbol: ALOX15B
• Synonyms: ALOX15B; arachidonate 15-lipoxygenase, type B; arachidonate 15 lipoxygenase, second type; arachidonate 15-lipoxygenase B; 15 LOX 2; 15-LOX-B; 15S-lipoxygenase; arachidonate 15-lipoxygenase 2; arachidonate omega(6) lipoxygenase; arachidonate 15-lipoxygenase type II; arachidonate 15-lipoxygenase, second type; 15-LOX-2
• Gene ID: 247
• mRNA Refseq: NM_001039130
• Protein Refseq: NP_001034219
• MIM: 603697
• UniProt ID: O15296
• Chromosome Location: 17p13.1
• Pathway: Arachidonic acid metabolism, organism-specific biosystem; Arachidonic acid metabolism, conserved biosystem; Eicosanoid Synthesis, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Selenium Pathway, organism-specific biosystem; Serotonergic synapse, organism-specific biosystem
• Function: arachidonate 15-lipoxygenase activity; iron ion binding; lipoxygenase activity; metal ion binding; oxidoreductase activity

Reviews

05/22/2022

By using this recombinant protein, the efficiency of my experiments was significantly improved, and unnecessary waste of time was also reduced.

06/21/2021

I appreciate the attention paid to product quality by this recombinant protein manufacturer, and their recombinant proteins are very stable, which makes our experiments smoother.

03/06/2020

The catalytic activity of this protein is very powerful and can effectively accelerate the process of chemical reactions, which brings great convenience to our experiments.

09/22/2019

The reproducibility of the Western blot was significantly improved using this recombinant protein.

Q&As

Does ALOX15B mutation cause autoimmune diseases?

Yes, certain ALOX15B gene mutations are closely associated with the onset of several autoimmune diseases.

What is the relationship between the expression level of ALOX15B in tumors and patient prognosis?

The expression level of ALOX15B in tumors is closely related to the treatment response and prognosis of patients.

What is the relationship between ALOX15B and diseases related to blood circulation system?

Whether ALOX15B is related to the occurrence of diseases related to the blood circulation system (such as heart disease, thrombosis, etc.) still needs further research.

What diseases will be caused if the ALOX15B gene is mutated?

Mutations in ALOX15B can lead to a variety of diseases, such as asthma and autoimmune diseases.

Does ALOX15B have application prospects in the treatment of skin diseases?

Yes, ALOX15B may be a target for the treatment of certain skin diseases.

How does the expression level of ALOX15B in patients with asthma correlate with the severity of the disease?

Studies have shown that the level of ALOX15B expression in asthma patients is positively correlated with the severity of the disease.

Does ALOX15B participate in the systemic inflammatory response of asthma?

Yes, ALOX15B can promote an increased systemic inflammatory response in patients with asthma.

Does ALOX15B have potential application value in the treatment of tumors?

Yes, some studies suggest that ALOX15B has the potential to be a new target for tumor therapy.

Is ALOX15B involved in tumor growth and development?

Yes, ALOX15B is associated with the occurrence, development and metastasis of various cancers.

What is the role of ALOX15B in the human body?

The enzyme encoded by ALOX15B is mainly involved in the metabolism of arachidonic acid (AA), which is closely related to biological processes such as inflammation and immune regulation.

What is the relationship between ALOX15B and asthma?

Many experimental studies have shown that ALOX15B is closely related to the pathogenesis and pathophysiological mechanism of asthma.

What is the role of ALOX15B in angiogenesis?

ALOX15B can inhibit angiogenesis and thus play a role in tumor growth and metastasis.

Is ALOX15B involved in the onset and progression of skin diseases?

Yes, ALOX15B is closely related to the onset and progression of many skin diseases (such as psoriasis, psoriasis, etc.).