Recombinant Human ALOX15B Protein, His-tagged ALOX15B-40H

Recombinant Human ALOX15B Protein, His-tagged


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Recombinant Human ALOX15B Protein, His-tagged

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Cat.No. : ALOX15B-40H
Product Overview : Recombinant human ALOX15B protein with a C-terminal His-tag was expressed in E. coli.
Description : This gene encodes a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. The encoded protein converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while metabolizing linoleic acid less effectively. This gene is located in a cluster of related genes and a pseudogene that spans approximately 100 kilobases on the short arm of chromosome 17. Alternatively spliced transcript variants encoding different isoforms have been described.
Source : E. coli
Species : Human
Tag : His
Molecular Mass : 76 kDa
Purity : ≥85% estimated by SDS-PAGE
Unit Definition : One unit is defined as the amount of enzyme required to produce 1 nmol of 15-HpETE per min at 30 centigrade in 50 mM Tris-HCl, pH 7.2, containing 0.003% Polysorbate 20, and 250 μM arachidonic acid.
Stability : ≥ 1 year
Storage : Store it at -80 centigrade
SDS-PAGE Analysis of ALOX15B :

Lane 1: MW Markers; Lane 2: 15-LO-2 (human recombinant) (1μg); Lane 3: 15-LO-2 (human recombinant) (2μg). Representative gel image shown; actual purity may vary between each batch.

Activity Assay :

15-LO-2 activity was determined using 50 μM arachidonic acid and was inhibited using 100 μM NDGA, a non-selective lipoxygenase inhibitor.

Gene Name : ALOX15B arachidonate 15-lipoxygenase type B [ Homo sapiens (human) ]
Official Symbol : ALOX15B
Synonyms : ALOX15B; arachidonate 15-lipoxygenase type B; 15-LOX-2; polyunsaturated fatty acid lipoxygenase ALOX15B; 15-LOX-B; 15S-lipoxygenase; arachidonate 15-lipoxygenase 2; arachidonate 15-lipoxygenase type II; arachidonate 15-lipoxygenase, second type; arachidonate omega(6) lipoxygenase; linoleate 13-lipoxygenase 15-LOb
Gene ID : 247
mRNA Refseq : NM_001039130
Protein Refseq : NP_001034219
MIM : 603697
UniProt ID : O15296

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