Recombinant Human CDK7 & CCNH & MNAT1 cell lysate
Cat.No. : | CDK7 & CCNH & MNAT1-539HCL |
Product Overview : | Human CDK7 & CCNH & MNAT1 Heterotrimer derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
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- Gene Information
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Source : | Baculovirus-Insect Cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | CDK7 cyclin-dependent kinase 7 [ Homo sapiens ]; CCNH cyclin H [ Homo sapiens ]; MNAT1 MNAT CDK-activating kinase assembly factor 1 [ Homo sapiens ] |
Official Symbol : | CDK7 & CCNH & MNAT1 |
Synonyms : | CAK1; HCAK; MO15; STK1; CDKN7; p39MO15; cyclin-dependent kinase 7; CAK; p39 Mo15; protein kinase; 39 KDa protein kinase; kinase subunit of CAK; CDK-activating kinase 1; serine/threonine kinase stk1; cell division protein kinase 7; serine/threonine protein kinase 1; serine/threonine-protein kinase 1; serine/threonine protein kinase MO15; homolog of Xenopus MO15 Cdk-activating kinase; TFIIH basal transcription factor complex kinase subunit; cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase); CAK; p34; p37; CycH; cyclin-H; CAK complex subunit; MO15-associated protein; CDK-activating kinase complex subunit; cyclin-dependent kinase-activating kinase complex subunit; MAT1; TFB3; CAP35; RNF66; CDK-activating kinase assembly factor MAT1; RING finger protein 66; RING finger protein MAT1; CDK7/cyclin-H assembly factor; cyclin G1 interacting protein; cyclin-G1-interacting protein; menage a trois 1 (CAK assembly factor); menage a trois homolog 1, cyclin H assembly factor |
Gene ID : | 1022; 902; 4331 |
mRNA Refseq : | NM_001799; NM_001199189; NM_001177963 |
Protein Refseq : | NP_001790; NP_001186118; NP_001171434 |
MIM : | 601955; 601953; 602659 |
UniProt ID : | P50613; P51948 |
Chromosome Location : | 5q12.1; 5q13.3-q14; 14q23 |
Pathway : | B Cell Receptor Signaling Pathway, organism-specific biosystem; Cyclin A/B1 associated events during G2/M transition, organism-specific biosystem; DNA Repair, organism-specific biosystem. Basal transcription factors, organism-specific biosystem; Cell Cycle, organism-specific biosystem; Cyclin A/B1 associated events during G2/M transition, organism-specific biosystem; Basal transcription factors, organism-specific biosystem; Cyclin E associated events during G1/S transition, organism-specific biosystem |
Function : | ATP binding; cyclin-dependent protein serine/threonine kinase activity; contributes_to DNA-dependent ATPase activity; contributes_to RNA polymerase II carboxy-terminal domain kinase activity; protein binding. contributes_to DNA-dependent ATPase activity; protein binding |
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◆ Lysates | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (6)
Ask a questionAt present, some drugs have been developed to act on CDK7, including some small molecule inhibitors and antibody drugs. These drugs can inhibit the activity of CDK7 or regulate its expression, thereby treating diseases associated with aberrant expression of CDK7.
Therapeutic strategies for CDK7 include inhibition of its activity, regulation of its expression, and gene therapy. For example, the development of small molecule inhibitors or antibody drugs against CDK7 to inhibit its aberrant activity in tumors, or the regulation of CDK7 expression through gene knockout or overexpression technologies.
The expression of CDK7 is affected by a variety of factors, including transcription factors, epigenetic modifications, and hormones. For example, the E2F transcription factor can promote the transcription of CDK7, while the histone methyltransferase EZH2 can regulate the cell cycle by inhibiting the transcription of CDK7.
Certain biomolecules can be used as molecular markers of CDK7, such as the mRNA or protein of CDK7 detected in biological samples such as blood and urine. These markers can be used for early diagnosis of disease, prognostic judgment, or monitoring of treatment efficacy.
The main role of CDK7 in the cell cycle is to activate CDK2 and CDK1, which in turn drive the cell from G1 to S phase and from G2 to M phase. It regulates cell cycle progression by phosphorylating a variety of substrates, including Rb protein and Wee1 kinase.
CDK7 activity can be detected by methods such as phosphowestern blotting, kinase activity assays, and cell cycle analysis. These methods can detect the phosphorylation level and cell cycle distribution of CDK7 to assess its activity status in cells.
Customer Reviews (3)
Write a reviewWe are very satisfied with the performance and quality of their products and will continue to buy them.
It has high catalysis, high safety and high expression level, which makes the experimental process more convenient and saves a lot of time for scientific research.
The fluorescence and absorption properties are very good.
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