Recombinant Human PDE1B cell lysate

• Cat.No.: PDE1B-627HCL
• Product Overview: Human PDE1B derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Species: Human
• Source: Insect Cells
• Tag: Non
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.

Gene Information

• Gene Name: PDE1B phosphodiesterase 1B, calmodulin-dependent [ Homo sapiens ]
• Official Symbol: PDE1B
• Synonyms: PDE1B; phosphodiesterase 1B, calmodulin-dependent; PDES1B; calcium/calmodulin-dependent 3,5-cyclic nucleotide phosphodiesterase 1B; cam-PDE 1B; 63 kDa Cam-PDE; calmodulin-stimulated phosphodiesterase PDE1B1; calcium/calmodulin-stimulated cyclic nucleotide phosphodiesterase; presumed 63kDa form of the type 1 cyclic nucleotide phosphodiesterase family known as PDE1B; PDE1B1
• Gene ID: 5153
• mRNA Refseq: NM_000924
• Protein Refseq: NP_000915
• MIM: 171891
• UniProt ID: Q01064
• Chromosome Location: 12q13
• Pathway: Ca-dependent events, organism-specific biosystem; CaM pathway, organism-specific biosystem; Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Calmodulin induced events, organism-specific biosystem; Cam-PDE 1 activation, organism-specific biosystem; DAG and IP3 signaling, organism-specific biosystem
• Function: 3,5-cyclic-AMP phosphodiesterase activity; calcium- and calmodulin-regulated 3,5-cyclic-GMP phosphodiesterase activity; calmodulin binding; calmodulin-dependent cyclic-nucleotide phosphodiesterase activity; hydrolase activity; metal ion binding