Recombinant Human RPS6KA6 cell lysate
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Cat.No. : | RPS6KA6-674HCL |
Product Overview : | Human RPS6KA6 / RSK4 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
Source : | Baculovirus-Insect Cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | RPS6KA6 ribosomal protein S6 kinase, 90kDa, polypeptide 6 [ Homo sapiens ] |
Official Symbol : | RPS6KA6 |
Synonyms : | RPS6KA6; ribosomal protein S6 kinase, 90kDa, polypeptide 6; ribosomal protein S6 kinase, 90kD, polypeptide 6; ribosomal protein S6 kinase alpha-6; RSK4; RSK-4; p90RSK6; p90-RSK 6; S6K-alpha 6; S6K-alpha-6; ribosomal S6 kinase 4; 90 kDa ribosomal protein S6 kinase 6; PP90RSK4; |
Gene ID : | 27330 |
mRNA Refseq : | NM_014496 |
Protein Refseq : | NP_055311 |
MIM : | 300303 |
UniProt ID : | Q9UK32 |
Chromosome Location : | Xq21.1 |
Pathway : | Activation of NMDA receptor upon glutamate binding and postsynaptic events, organism-specific biosystem; Axon guidance, organism-specific biosystem; CREB phosphorylation through the activation of Ras, organism-specific biosystem; Cytoplasmic Ribosomal Proteins, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Insulin Signaling, organism-specific biosystem; L1CAM interactions, organism-specific biosystem; |
Function : | ATP binding; magnesium ion binding; nucleotide binding; protein kinase activity; protein serine/threonine kinase activity; |
◆ Recombinant Protein
Rps6ka6-5612M | Recombinant Mouse Rps6ka6 Protein, Myc/DDK-tagged | +Inquiry |
RPS6KA6-2436H | Recombinant Human RPS6KA6, His-tagged | +Inquiry |
RPS6KA6-3329H | Recombinant Human RPS6KA6, GST tagged | +Inquiry |
RPS6KA6-30418TH | Recombinant Human RPS6KA6 | +Inquiry |
RPS6KA6-427H | Recombinant Human RPS6KA6, GST-tagged, Active | +Inquiry |
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RPS14 RPS27A RPS3 RPS6KA1 RPS6KA2 RPS6KA3 RPS6KA5 RPS6KA6 RPS6KB1 RPS6KB2 RPS6 RPS15 RPS17 RPS19 RPS2 RPS23 RPS27 RPS5 RPS7 RPS6KA4 RPS11 RPS20 RPS28 RPS29 RPS4X RPS6KC1 RPS8 RPS9 RPS24 RPS13 RPS26 RPS27L RPS18 RPS19BP1 RPS21 RPS25 RPS6KL1 RPS4Y1 RPS3A RPS15A RPS10 RPS12 Rps16 RPS17L
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