Recombinant Human SMYD2 cell lysate
Cat.No. : | SMYD2-705HCL |
Product Overview : | Human SMYD2 / KMT3C derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
- Related Products
Source : | Baculovirus-Insect Cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | SMYD2 SET and MYND domain containing 2 [ Homo sapiens ] |
Official Symbol : | SMYD2 |
Synonyms : | SMYD2; SET and MYND domain containing 2; N-lysine methyltransferase SMYD2; HSKM B; KMT3C; ZMYND14; lysine N-methyltransferase 3C; histone methyltransferase SMYD2; zinc finger, MYND domain containing 14; SET and MYND domain-containing protein 2; HSKM-B; MGC119305; |
Gene ID : | 56950 |
mRNA Refseq : | NM_020197 |
Protein Refseq : | NP_064582 |
MIM : | 610663 |
UniProt ID : | Q9NRG4 |
Chromosome Location : | 1q32.3 |
Pathway : | p53 pathway, organism-specific biosystem; |
Function : | RNA polymerase II core binding; histone methyltransferase activity (H3-K36 specific); metal ion binding; methyltransferase activity; p53 binding; protein binding; protein-lysine N-methyltransferase activity; transferase activity; zinc ion binding; |
Products Types
◆ Recombinant Protein | ||
SMYD2-2063H | Recombinant Human SMYD2 Protein, MYC/DDK-tagged | +Inquiry |
SMYD2-049H | Active Recombinant Human SMYD2 Protein, His/SUMO-tagged | +Inquiry |
SMYD2-8507M | Recombinant Mouse SMYD2 Protein, His (Fc)-Avi-tagged | +Inquiry |
SMYD2-019H | Active Recombinant Human SMYD2 Protein, His/SUMO-tagged | +Inquiry |
Smyd2-5986M | Recombinant Mouse Smyd2 Protein, Myc/DDK-tagged | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Q&As
- Reviews
Q&As (5)
Ask a questionInvestigations into small molecules and inhibitors targeting SMYD2 are underway, with potential applications in cancer and cardiovascular disease treatment.
Monitoring SMYD2 levels may provide insights into the progression of cardiovascular diseases, aiding in early diagnosis and treatment.
Research suggests that SMYD2 may play a role in neurodegenerative diseases, and exploring its modulation could offer new avenues for therapeutic intervention.
SMYD2 is involved in various cellular processes, including cell cycle regulation, DNA repair, and inflammation, making it a versatile protein with potential implications in multiple diseases.
SMYD2 has been linked to cardiac hypertrophy and heart failure, suggesting its potential as a therapeutic target in the context of cardiovascular diseases.
Customer Reviews (3)
Write a reviewIts compatibility with various methodologies further enhances its value as a research tool, making it a go-to choice for investigating SMYD2 protein function and molecular interactions.
The versatility of the SMYD2 protein extends beyond these applications, enabling its use in a wide range of experimental techniques.
the SMYD2 protein stands out for its exceptional quality and its ability to fulfill my experimental requirements.
Ask a Question for All SMYD2 Products
Required fields are marked with *
My Review for All SMYD2 Products
Required fields are marked with *
Inquiry Basket