Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Recombinant Human SMYD2 cell lysate

Cat.No. : SMYD2-705HCL
Product Overview : Human SMYD2 / KMT3C derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
  • Specification
  • Gene Information
  • Related Products
Source : Baculovirus-Insect Cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : SMYD2 SET and MYND domain containing 2 [ Homo sapiens ]
Official Symbol : SMYD2
Synonyms : SMYD2; SET and MYND domain containing 2; N-lysine methyltransferase SMYD2; HSKM B; KMT3C; ZMYND14; lysine N-methyltransferase 3C; histone methyltransferase SMYD2; zinc finger, MYND domain containing 14; SET and MYND domain-containing protein 2; HSKM-B; MGC119305;
Gene ID : 56950
mRNA Refseq : NM_020197
Protein Refseq : NP_064582
MIM : 610663
UniProt ID : Q9NRG4
Chromosome Location : 1q32.3
Pathway : p53 pathway, organism-specific biosystem;
Function : RNA polymerase II core binding; histone methyltransferase activity (H3-K36 specific); metal ion binding; methyltransferase activity; p53 binding; protein binding; protein-lysine N-methyltransferase activity; transferase activity; zinc ion binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (5)

Ask a question
Are there any drugs targeting SMYD2 in the clinical pipeline? 07/30/2020

Investigations into small molecules and inhibitors targeting SMYD2 are underway, with potential applications in cancer and cardiovascular disease treatment.

In what ways can SMYD2 be utilized in the diagnosis of cardiovascular disorders? 06/29/2020

Monitoring SMYD2 levels may provide insights into the progression of cardiovascular diseases, aiding in early diagnosis and treatment.

Can SMYD2 be used as a therapeutic target for neurodegenerative disorders? 03/26/2020

Research suggests that SMYD2 may play a role in neurodegenerative diseases, and exploring its modulation could offer new avenues for therapeutic intervention.

How does SMYD2 influence cellular processes beyond cancer and cardiac diseases? 07/15/2018

SMYD2 is involved in various cellular processes, including cell cycle regulation, DNA repair, and inflammation, making it a versatile protein with potential implications in multiple diseases.

How does SMYD2 contribute to cardiac disease? 10/05/2016

SMYD2 has been linked to cardiac hypertrophy and heart failure, suggesting its potential as a therapeutic target in the context of cardiovascular diseases.

Customer Reviews (3)

Write a review
Reviews
01/16/2018

    Its compatibility with various methodologies further enhances its value as a research tool, making it a go-to choice for investigating SMYD2 protein function and molecular interactions.

    03/20/2017

      The versatility of the SMYD2 protein extends beyond these applications, enabling its use in a wide range of experimental techniques.

      08/31/2016

        the SMYD2 protein stands out for its exceptional quality and its ability to fulfill my experimental requirements.

        Ask a Question for All SMYD2 Products

        Required fields are marked with *

        My Review for All SMYD2 Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends