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Recombinant Mouse NTRK2 cell lysate

Cat.No.: NTRK2-1843MCL
Product Overview: Mouse NTRK2 / TrkB derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
Source: Human cells
Species: Mouse
Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability: Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name: Ntrk2 neurotrophic tyrosine kinase, receptor, type 2 [ Mus musculus ]
Official Symbol: NTRK2
Synonyms: NTRK2; neurotrophic tyrosine kinase, receptor, type 2; BDNF/NT-3 growth factors receptor; trk-B; GP145-TrkB/GP95-TrkB; trkB tyrosine kinase; Tkrb; trkB; AI848316; C030027L06Rik;
Gene ID: 18212
mRNA Refseq: NM_001025074
Protein Refseq: NP_001020245
Pathway: Activation of TRKA receptors, organism-specific biosystem; MAPK signaling pathway, organism-specific biosystem; MAPK signaling pathway, conserved biosystem; NGF signalling via TRKA from the plasma membrane, organism-specific biosystem; NGF-independant TRKA activation, organism-specific biosystem; Neurotrophin signaling pathway, organism-specific biosystem; Neurotrophin signaling pathway, conserved biosystem;
Function: ATP binding; brain-derived neurotrophic factor binding; brain-derived neurotrophic factor binding; brain-derived neurotrophic factor-activated receptor activity; brain-derived neurotrophic factor-activated receptor activity; kinase activity; neurotrophin receptor activity; neurotrophin-3 binding; neurotrophin-3 binding; neurotrophin-4/5 binding; nucleotide binding; protein binding; protein homodimerization activity; protein kinase activity; protein tyrosine kinase activity; receptor activity; transferase activity; transferase activity, transferring phosphorus-containing groups; transmembrane receptor protein tyrosine kinase activity;

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