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Recombinant Human SECTM1 cell lysate

Cat.No. : SECTM1-1946HCL
Product Overview : Human SECTM1 / K12 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : SECTM1 secreted and transmembrane 1 [ Homo sapiens ]
Official Symbol : SECTM1
Synonyms : SECTM1; secreted and transmembrane 1; secreted and transmembrane protein 1; K12; K12 protein; type 1a transmembrane protein;
Gene ID : 6398
mRNA Refseq : NM_003004
Protein Refseq : NP_002995
MIM : 602602
UniProt ID : Q8WVN6
Chromosome Location : 17q25
Function : cytokine activity; signal transducer activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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Does SECTM1 protein interact with other proteins? 09/08/2023

The SECTM1 protein may interact with other proteins to fulfill its function. These interactions can involve various types of protein-protein interactions like electrostatic interactions, hydrogen bonding, etc. Understanding these interactions can help unravel the mechanism of action of the SECTM1 protein in cells.

How to design experiments to study the structure and function of SECTM1 protein? 06/05/2023

Studying the structure and function of the SECTM1 protein may require the design of a series of experiments, including biochemical and cell biology experiments. For example, its structure can be studied by nuclear magnetic resonance or X-ray crystal diffraction, and its function can be studied by gene knockout or knockdown techniques.

How to evaluate the potential of the SECTM1 protein as a drug target? 05/01/2023

Evaluating the potential of the SECTM1 protein as a drug target can involve several aspects, including its expression levels in organisms, its role in biological processes, and its changes in disease. In addition, factors such as ease of drug development for this protein and potential drug side effects need to be considered.

What is the pathological significance of SECTM1 protein? 04/08/2023

SECTM1 protein may play an important role in the development and progression of certain diseases. For example, changes in its expression levels may be related to the occurrence and development of tumors. Studying the pathological significance of the SECTM1 protein can help develop therapeutic strategies against this protein.

What are the effects of mutations or modifications in the SECTM1 protein on organisms? 06/29/2020

Mutations or modifications in the SECTM1 protein may affect its function and thus affect the organism. The specific effects may vary depending on the type and location of the mutation or modification and may involve various biological processes, such as growth and development, immune response, etc.

How is the expression level of SECTM1 protein regulated? 06/16/2020

The expression level of SECTM1 protein may be regulated by a variety of factors, including gene transcription, translation, post-transcriptional modifications, etc. Studying these regulatory mechanisms can help to understand the expression patterns of SECTM1 protein in organisms and its impact on biological processes.

Customer Reviews (3)

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Reviews
03/08/2023

    I've used a lot of brands, but this one is one of my favorites.

    04/15/2022

      The storage conditions of SECTM1 are simple, which is convenient for long-term storage and use.

      12/07/2020

        The expression level and stability are good, and will be repurchased.

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