Recombinant Human CD276 cell lysate
Cat.No. : | CD276-1959HCL |
Product Overview : | Human CD276 / B7-H3 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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Source : | Human cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | CD276 CD276 molecule [ Homo sapiens ] |
Official Symbol : | CD276 |
Synonyms : | CD276; CD276 molecule; CD276 antigen; B7 H3; B7H3; B7RP 2; B7 homolog 3; costimulatory molecule; B7-H3; B7RP-2; 4Ig-B7-H3; |
Gene ID : | 80381 |
mRNA Refseq : | NM_001024736 |
Protein Refseq : | NP_001019907 |
MIM : | 605715 |
UniProt ID : | Q5ZPR3 |
Chromosome Location : | 15q23-q24 |
Pathway : | Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; |
Function : | protein binding; receptor binding; |
Products Types
◆ Lysates | ||
CD276-001MCL | Recombinant Mouse CD276 cell lysate | +Inquiry |
CD276-826RCL | Recombinant Rat CD276 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionCD276 has been associated with autoimmune diseases and certain inflammatory conditions. Exploring its role in these diseases may open avenues for therapeutic interventions.
Research suggests that CD276 expression levels may serve as a diagnostic biomarker for certain cancers. Detection methods, such as immunohistochemistry, are being explored to assess CD276 levels in tumor tissues.
CD276 can modulate the tumor microenvironment by suppressing immune responses, promoting angiogenesis, and supporting tumor cell survival. Understanding these interactions is crucial for developing targeted therapies.
Yes, several clinical trials are underway to evaluate the safety and efficacy of CD276-targeted therapies in different cancer types. These trials aim to assess the impact on patient outcomes and potential side effects.
CD276-targeted therapy focuses on modulating the immune response against cancer cells, whereas traditional treatments like chemotherapy and radiation target rapidly dividing cells, including both healthy and cancerous cells.
Customer Reviews (3)
Write a reviewIt has consistently demonstrated high sensitivity and accuracy in detecting and quantifying target molecules, allowing for precise and reliable measurement of analytes in my experimental samples.
Its versatility and reliability make it an excellent choice for research applications, and I highly recommend it to fellow scientists.
The sensitivity and specificity of the CD276 Protein have consistently delivered robust and consistent results, enhancing the efficiency and accuracy of my experimental workflows.
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