"SEMA6A" Related Products

Recombinant Human SEMA6A cell lysate

Cat.No.: SEMA6A-2081HCL
Product Overview: Human Semaphorin-6A / SEMA6A derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
Source: Human cells
Species: Human
Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability: Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name: SEMA6A sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A [ Homo sapiens ]
Official Symbol: SEMA6A
Synonyms: SEMA6A; sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A; SEMAQ; semaphorin-6A; HT018; KIAA1368; SEMA; sema VIa; SEMA6A1; SEMA6A-1; semaphorin VIA; semaphorin 6A-1; semaphorin-6A-1; VIA;
Gene ID: 57556
mRNA Refseq: NM_020796
Protein Refseq: NP_065847
MIM: 605885
UniProt ID: Q9H2E6
Chromosome Location: 5q23
Pathway: Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Other semaphorin interactions, organism-specific biosystem; Semaphorin interactions, organism-specific biosystem;
Function: protein binding; receptor activity; semaphorin receptor binding;

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