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Recombinant Mouse EPCAM cell lysate

Cat.No. : EPCAM-2143MCL
Product Overview : Mouse EpCAM / TROP1 / CD326 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Human cells
Species : Mouse
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Epcam epithelial cell adhesion molecule [ Mus musculus ]
Official Symbol : EPCAM
Synonyms : EPCAM; epithelial cell adhesion molecule; mEGP314; protein 289A; Trop-1 protein; lymphocyte antigen 74; epithelial glycoprotein 314; panepithelial glycoprotein 314; tumor-associated calcium signal transducer 1; EGP; Ly74; gp40; CD326; EGP-2; TROP1; Egp314; Ep-CAM; Tacsd1; GA733-2; Tacstd1;
Gene ID : 17075
mRNA Refseq : NM_008532
Protein Refseq : NP_032558

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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In which types of tissues is EpCAM predominantly expressed? 12/07/2022

EpCAM is highly expressed in epithelial tissues and some cancer cells.

How does EpCAM contribute to cell adhesion? 08/21/2022

It has a controversial role in cell adhesion, possibly contributing to both cell-cell adhesion and anti-adhesive properties.

What is the primary function of EpCAM in cells? 04/16/2022

EpCAM primarily functions in cell signaling, proliferation, and migration.

What role does EpCAM play in cancer progression? 03/19/2022

It often has a pro-tumorigenic role, promoting cancer cell proliferation, migration, and invasion.

How is EpCAM used as a biomarker in cancer diagnosis? 03/28/2021

EpCAM is used as a biomarker to detect and isolate circulating tumor cells.

What therapeutic strategies are being explored involving EpCAM? 11/23/2018

Strategies include developing EpCAM-targeted therapies for cancer treatment.

Are there known genetic alterations in EpCAM associated with specific health conditions? 11/07/2018

Genetic alterations in EpCAM are linked to some hereditary cancer syndromes.

Customer Reviews (3)

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Reviews
02/13/2022

    Reliable for assays, satisfied customer.

    09/09/2019

      High-quality product, consistent results.

      04/03/2019

        Quick delivery, product as described.

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