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Recombinant Human REG1A cell lysate

Cat.No.: REG1A-2497HCL
Product Overview: Human REG1A / PSPS derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
Source: Human cells
Species: Human
Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability: Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name: REG1A regenerating islet-derived 1 alpha [ Homo sapiens ]
Official Symbol: REG1A
Synonyms: REG1A; regenerating islet-derived 1 alpha; REG, regenerating islet derived 1 alpha (pancreatic stone protein, pancreatic thread protein); lithostathine-1-alpha; pancreatic stone protein; pancreatic thread protein; PSP; PSPS; PSPS1; PTP; protein-X; REG-1-alpha; lithostathine 1 alpha; regenerating protein I alpha; islet cells regeneration factor; pancreatic stone protein, secretory; islet of langerhans regenerating protein; regenerating islet-derived protein 1-alpha; P19; REG; ICRF; MGC12447;
Gene ID: 5967
mRNA Refseq: NM_002909
Protein Refseq: NP_002900
MIM: 167770
UniProt ID: P05451
Chromosome Location: 2p12
Pathway: Insulin Signaling, organism-specific biosystem; Type II interferon signaling (IFNG), organism-specific biosystem;
Function: binding; growth factor activity; sugar binding;

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